The two techniques that were used to create a DNA profile in this experiment were PCR and gel electrophoresis. The PCR is used to amplify the several DNA samples and gel electrophoresis is performed to separate the DNA fragments according to their size. [6] In the first part of the experiment‚ PCR amplification of the DNA templates was performed and the products obtained were used to perform gel electrophoresis. The process of PCR allows for the amplification of the DNA samples and the components
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In order to read DNA‚ it must be sequenced. This sequencing uses electrophoresis‚ a technique that separates sections of DNA that differ by a base. Electrophoresis used to be done manually‚ but was error prone and time consuming. Now‚ automatic sequencing machines are used. A technician begins the process by pouring gel between two glass plates that are set less than half a millimeter apart. After the gel is set up‚ DNA is put into each of the ninety-six lanes. The DNA sections then move through
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DNA extraction and purification is an essential tool in understanding the biological basis and significance of a eukaryotic cell. Its role is pivotal to numerous scientific applications ranging from basic science research to applied research. Hence‚ DNA is found in all living organisms. Fragaria x ananassa‚ also known as a strawberry has been widely used as one of the many biological models in studying DNA structures due to its accessibility. Strawberries are octoploid which contain large genomes
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The purpose of module E is to learn several DNA techniques in the lab including DNA purification with solubility and absorption‚ plasmid transfection of E.coli‚ colony screening by PCR and quantitative PCR. First part of the experiment E1 show the purification method of DNA through solubility. E. coli lysate mixed organic solvents to purify the DNA present in solution. First‚ the lysate was mixed with phenol/chloroform‚ then vortexed‚ and centrifuged. We extracted the aqueous layer and combined
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How does movie Gattaca relate to biology? The movie Gattaca directed by Andrew Niccol is about “a genetically inferior man assumes the identity of a superior one in order to pursue his lifelong dream of space travel”. During the process (to become as same as Jerome Morrow‚ the superior)‚ Vincent goes through lots of different operations‚ including physical and genetic operations. As we watched it and saw the operations and the doctors in the beginning of the movie‚ we connected happenings to the
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Low Molecular Weight Heparin Paper Low molecular weight heparin is typically used for patients who need to be treated for deep vein thrombosis. Deep vein thrombosis (DVT) is a blood clot (thrombus) in a deep vein usually in the legs. These clots are dangerous because they can break loose‚ travel through the bloodstream to the lungs‚ and block blood flow in the lungs (pulmonary embolism). There are many reasons why clots form in a patient. Mainly
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Research Paper on E.Coli Paper Summary: This article is written about the issues regarding E.coli being found in meat that is being sold to consumers in stores nation wide. Each section looks at a different department and what efforts they are making to try and prevent further cases of E.coli in meat products. Culprit in Article: the Company that is considered the culprit in this article and is the one who has been accused for the selling of the frozen hamburger that paralyzed Ms. Smith from
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Abstract:Conjugation is a natural occurring process that involves the transfer of DNA from one cell into another through a physical connection between the cells. In the following experiment‚ two strains of Escherichia coli bacterial cells (donor F’lac+strs and recipient F-lac-strr) underwent conjugation to produce a transconjugant strain (F’lac+strr). MAC plates and streptomycin were utilized to determine if conjugation had occurred. When plated‚ the donor colonies appeared red and the recipient
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The Phenomenon that is Genetic Transformation Matt Kimmel November 17‚ 2011 Bio 121-012 Introduction Genetic transformation is when the genetic makeup of an organism is altered by it receiving external genetic material (Barnhart and Hopper‚ 2011). Bacterial transformation was first seen during an experiment by Fredric Griffith in 1928. In the experiment there were two strains of bacteria‚ a virulent strain‚ and non-virulent strain. Virulent simply means disease causing‚ and therefore non-virulent
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Transformation of E. coli by plasmid DNA 1. Table showing the results from the selective plates |Plate |Plasmid |Contents of plates |Number of colony | | | | |White |Blue | |1 |Ligation mixture |Ampiclillin + X- gal + IPTG |10 |0
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