Restriction Fragment Length Polymorphism (RFLP) Restriction fragment length polymorphism (RFLP) analysis was one of the first techniques to be widely used for detecting variation at the DNA sequence level. The principle behind the technology rests on the possibility of comparing band profiles generated after restriction enzyme digestion in DNA molecules of different individuals. Diverse mutations that might have occurred affect DNA molecules in different ways‚ producing fragments of variable lengths
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The hypoglycaemic and antihyperglycaemic effects of methanol extract of the leaves of Cleome rutidosperma (Cr) DC (Family: Capparidaceae) was investigated in Wistar rats. Fifty normoglycaemic male rats (120g-200g) were divided into groups A (hypoglycaemic study; n=20) and B (antihyperglycaemic study; n=30). The studies had one control group and three groups administered with Cr (100‚ 200 or 400mg/kg) respectively. Group B had two additional groups of diabetic-untreated rats and glibenclamide-treated
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8 April‚ 2013 Who Hit Reveille? Reveille was hit by a cyclist while walking with her handler‚ but the suspect got away. However‚ the cadet was able to grab a notebook from the cyclist as he tried to stop him from escaping. A code maroon was sent out‚ in search of a cyclist on a maroon and white bike wearing blue jeans. Two men who fit the description were taken to the campus police for further investigation. A series of forensic tests were conducted to determine which‚ if any‚ of these two cyclists
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Rob Knight’s TED talk‚ “How our microbes make us who we are‚” addresses the role of microbes in our development and health along with our genetic makeup. He discusses many important microbial roles in the human body‚ including those discovered by the Human Microbiome Project. Knight has incorporated the basis of the Human Microbiome Project to essentially map out the microbes of the human body on a worldwide spectrum into the oral‚ skin‚ vaginal‚ and fecal communities. In his project‚ Knight indicates
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Purification of Infliximab[3] begins at Stage 1 with the filtration of the harvest material using a spin filter. The harvest is then clarified and undergoes 3 main chromatographic steps. Firstly‚ at Stage 2‚ the direct protein capture by Protein A affinity chromatography helps to remove a large majority of the impurities whicn include viruses and media components. At Stage 3‚ prior to cation exchange chromatography‚ the purified material in the eluted stream is then subjected to freezing‚ thawing
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II. HCV Sample Dilution and Inactivation From each sample 225 µL was transferred to a microcentrifuge tube‚ which containing 25 µL of Triton X-100 Solution; the tubes were Incubated 30 minutes at 37ºC‚ and then the samples were incubated at 56ºC for 30 min in water path (for dissotiation of HCV core antigen-antibody complex). Assay procedure 1. All reagents were leaved to reach room temperature before use‚ and then shacked thoroughly with no foam. 2. The number of strips required for the test
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The tissue samples were visualized using a Leica microscope (Leica Microsystems‚ Wetzlar‚ Germany)‚ and the Leica image manager software to enhance and photograph the sections. All sections were photographed and epidermal thickness was measured 50 places per section and the average epidermal thickness was calculated pr section (Fig. S5). The measurements were made on sections photographed at 10X enhancement. All sections were blinded to the observer during measurements. Strengths: Good method
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Genomic Library A genomic library is a collection of recombinant vectors that represent the entire genome of an organism. Below is a brief outline of how to create the genomic library: 1. We isolated Vibrio fischeri DNA to pGEM genomic DNA using a phenol chloroform purification method. 2. Vibrio fischeri DNA and pGEM DNA was cut with restriction enzyme Sal I to prepare both for ligation. 3. Vibrio fischeri and pGEM DNA were ligated with T4 ligase. 4. E.coli DH5α competent cells were prepared
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DNA fingerprinting is a method that compares the fragments of DNA. DNA fingerprinting was first invented to detect the presence of genetic diseases. Today‚ DNA fingerprinting is used in different ways. DNA is analyzed using a Southern Blot‚ which allows scientists to observe the base pair patterns. DNA fingerprinting can be used in a few different ways. First‚ to find out if the child belongs to a person DNA fingerprinting may be used. When a child is born‚ it inherits the VNTR’s from the father
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Dorothy Crowfoot (later called Dorothy Crowfoot Hodgkin) was born on May 12‚ 1910 in Cairo‚ Egypt to John Winter Crowfoot and Grace Mary Hood Crowfoot. John was a school inspector that worked for the Egyptian Education Service and later became the Director of Education and Director of Antiquities in Sudan. He retired in 1926 and focused on archaeology and later became the Director of Jerusalem’s British School of Archaeology. Grace Mary was botanist and illustrated the different flora found in Sudan
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