DNA bender: Cren7 & Sul7 DNA benders can introduce a bend in the DNA (Luijsterburg et al.‚ 2008). Many bends in the DNA automatically provide compaction of the DNA. Two important DNA bending proteins in crenarchaea are Cren7 and Sul7 (Driessen et al.‚ 2013). They are similar in structure‚ but they have different DNA binding regions (Zhang et al.‚ 2015). Cren7 and Sul7 can be methylated at several lysine residues (Guo‚ 2007). This PTM might be to regulate gene expression (Feng et al.‚ 2010)‚ although
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The procedure was taken from “From Drosophila cDNA in E. coli plasmid to homologous human proteins” lab manual (4). - Colony Picking: Two E. coli colonies were grown on agar plates and treated with ampicillin. They contained the plasmid with genes for ampicillin resistance and Drosophila cDNA sequence. - Plasmid Isolation: We used the QuickLyse Miniprep Plasmid DNA purification systems to isolate the plasmid DNA. Indeed‚ the bacterial cells were removed from the liquid broth and were resuspended
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Forensic anthropologists have been under the microscope of whether their ability to identify race is accurate or not. For many centuries‚ there was has been a debate whether race can have biological variations (Ousley‚ Jantz‚ & Freid‚ D‚ 2009‚ p.68). For many centuries there has been a debate whether race can have biological variations (Ousley‚ Jantz‚ & Freid‚ D‚ 2009‚ p.68). Forensic anthropologists are called in to help identify physical characteristics which are then used to determine the physical
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Introduction and Missing persons: Last week a couple came across a human skull that was found in a park. Police were contacted and it appeared to be two human skeletons. So as a team of forensic anthropologist we studied skeleton B to see whether or not it would match with the following missing people. Stacey Jackson or Regina Rashad. Stacey Jackson is a 26 year old grocery clerk she’s been missing for 3 months. Two witnesses remember seeing her on the path near the park the same day she had disappeared
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Introduction When a bacterium integrates a piece of DNA into its genome‚ bacterial transformation has occurred. In this experiment bacterial transformation will be done using calcium chloride/heat shock. This is done by incorporating the plasmids into chemically competent cells that were made permeable by the calcium chloride solution and heat shock. In 1928‚ Frederick Griffith‚ a physician from London‚ was he first person to experiment with bacterial transformation. He permanently transformed a
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The FDA’s recent approval of transgenic (genetically engineered) salmon gave biotech firm‚ AquaBounty the go ahead to sell its genetically engineered Atlantic salmon. This decision set a precedent as the first genetically altered animal product approved for human food in the United States. Approval opens the door for other transgenic food products. Transgenic foods are a hotly debated topic. The approval met with a mix of elation from proponents and unbridled fury from some environmental and consumer
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Ligation is the process of joining two DNA fragments or other molecules by a phosphate ester linkage with the action of enzyme. Ligation of DNA is process that involve the DNA of interest is inserted into the plasmid. This 3’-hydroxyl of DNA terminus are joined together with 5’-phosphoryl of another by phosphodiester bond. The ligation of DNA fragments usually performed by using T4 DNA ligase. All the reaction components such as ligation buffer‚ DNA insert‚ pGEM®-T‚ and T4 DNA ligase is mixed by
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Since the beginning of time‚ the human race has attempted to perfect our lives‚ constantly adapting‚ and learning‚ and changing our environment to suit our needs. we have been changing the world dramatically in a large variety of environments from the atmosphere to the rainforests‚ and soil in which the trees grow. Up to this point we have been manipulating our environment in nearly any way we can imagine‚ making ourselves the dominant race on the earth‚ but perhaps we should be aware of how far
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Genetic transformation happens when an organism is altered by the introduction of new genetic information which is merged into the organism’s genome. Bacterial transformation is a type of genetic transformation that was used in lab and mainly used due to the single celled nature of bacteria. In this lab‚ the engineered pGLO plasmid is integrated into E. Coli bacteria‚ and adds the genes which code for the proteins GFP in the modified bacteria’s genome (Hanahan‚ Studies on transformation of Escherichia
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Bacterial Genetics Worksheet 1. Fill in: Transformation Transduction Conjugation a. Transformation _ naked DNA is involved b. Transduction ____faulty head stuffing may occur c. Conjugation_____ involves exconjugants d. Transduction____ adsorption and injection of genetic material e. Transformation__ competent cells required f. Conjugation ____ an F plasmid encodes attachment proteins g. Conjugation _____used to construct the E. coli minute map h. Conjugation_____
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