In silico search for the identification of HXK1 and MAKR6 in P. scoparia So as to know all the members of HXK1 and MAKR6 in gene wild almond‚ these HXK1 and MAKR6 sequences in regard to A. thaliana and Oryza sativa and DATF (Http://datf.cbi.pku.cn) [72] database and DRTF (http://drtf.cbi.pku.cn) [72] database‚ respectively. In order to look for HXK1 and Makr6 genes in P. scoparia‚ the HXK1 and MAKR6 domain was used together with BLASTP and TBLATN [73] acquired from the NCBI information base (http://www
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Purpose: Restriction enzymes cut DNA at a certain palindromic sequence. Three samples of lamda DNA set up to be cut with restriction enzymes PstI‚ EcoRI‚ or HindDIII. There were also two more samples‚ one of these samples was not mixed with any restriction enzyme and the other was a marker‚ which used an enzyme which creates fragments with a known number of base pairs used to create a standard curve. All five samples were put through agarose gel electrophoresis in order to estimate the amount of
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Detection of plasmids in arsenic (As) resistant bacteria isolated from As-contaminated groundwater Mahima Rani and Pinaki Sar* Department of Biotechnology‚ Indian Institute of Technology‚ Kharagpur‚ West Bengal–721302‚ India mahima06bt11@gmail.com‚ Ph: +91 8348523016 Abstract Role of plasmids in conferring resistance to several heavy metals and antibiotics to naturally occurring bacteria is well known. In contaminated environments‚ presence of metal resistant genes on plasmids often provide
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November 25‚ 2012 The Effect of the pGLO Plasmid on Genetic Transformation of E.coli by Heat Shock Introduction Genetic transformation is the genetic alteration of a cell resulting from the direct uptake‚ incorporation and expression of exogenous genetic material l(exogenous DNA) from its surroundings and taken up through the cell membranes. This was first demonstrated in 1928 by Bacteriologist Frederick Griffith (Lederberg 2000).A plasmid is a small circular piece of DNA that contains important
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Isolation‚ Expression and Cloning of Lactate Dehydrogenase Gene from Lactobacillus bulgaricus Lactobacillus bulgaricus is used for industrial production of yogurt mainly because of his fermentative capability. In this experiment‚ the gene encoding the enzyme lactate dehydrogenase [ldhA] from the bactaria was isolated and cloned. The expressed ldhA gene was inserted into pET28b plasmid vector. The resulting recombinant pET28b-LdhA expression vector was then transformed after introduction into E.
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Materials and Methods Growing the G Strain and Preparing the GCE (rGFP Crude Extract): To grow the bacterial culture‚ use 10 ml of liquid LB growth media for incubation. 500 ml of the bacterial culture is allowed to grow overnight at 37°C. It is later shaken vigorously to increase the OD600 to 0.5‚ which means that time equals zero. At time zero‚ 1 mL of the culture is transferred into a 1.5 mL centrifuge tube and centrifuged for 5-10 minutes to obtain a pellet. The supernatant should be discarded
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Modeling bacterial growth is important in maximizing the efficiencies of biological processes. Although there are many different methods of modeling bacterial growth‚ this experiment focuses on the Monod equations. However‚ in order to use the Monod equations‚ the maximum growth rate and Monod constant must be found. Here we show how the maximum growth rate and Monod constants can be obtained for Escherichia coli using M9 media in a bioreactor at 37 °C and 500 RPM. The maximum growth rate is also
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Objectives Protein Isolation: Protein isolation for a western blot uses detergents and mechanical force to separate seeded cells from their container. Eukaryotic cells are attached to the surface of a flask by cadherins. In the past‚ we’ve separated the cells from the flask by breaking these bonds with a protease‚ but in order to keep the proteins intact‚ a different method needs to be used to extract the proteins. In protein extraction for a western blot‚ we use detergents to lyse the membrane
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DNA Cloning PCB3063L Section DNA cloning refers to the process of making multiple copies of a DNA fragment. For the past weeks we have conducted a set of experiments that allow us to clone a specific gene in drosophila. First we started by the process of DNA extraction‚ which allowed us to isolate the genomic DNA from D. Melanogaster. This process requires the use of lysis in other to extract the DNA and RNA. After extracting the DNA‚ we it is important to use
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The team of forensic anthropologists ran a gel electrophoresis with DNA from Skeleton 3 and two missing persons‚ Julia Ly and Teresa Chen to help in DNA identification. This process would allow restriction enzymes to cut by a specific restriction site and run through the gel‚ where the DNA fragments would move from the negative side to the positive side of the gel due to the negative charge of the phosphate group in DNA. The smaller the DNA fragments‚ the further they move down the gel. As mentioned
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