Clustered regularly interspaced short palindromic repeats (CRISPR) is a new technology that allows for DNA to be altered in order to make genetic changes to human cells. This is important in the Science department because CRISPR is a much easier‚ less complicated way‚ more secure and less expensive way to edit genes. CRISPR has two main parts; an enzyme that acts like a scissor that cuts two strands of DNA at a specific location in order for DNA to be added or removed‚ and a piece of RNA that makes
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FISH FISH stands for Fluorescence in situ hybridization. FISH is a technique in the lab used to see where a DNA sequence or certain gene is located in an individual’s genome. This allows scientists to look for genetic conditions caused by alterations in chromosomes. FISH can be used to find distinct features in DNA for genetic counseling‚ species identification‚ and medicine. It can also be used to find certain RNA points in cells and tissue samples. FISH is used on blood samples or any other cell
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Extraction and Analysis of Plasmid DNA from E. coli Cells Introduction A plasmid is an extra-chromosomal element‚ often a circular DNA. Since a plasmid is by definition an extra-chromosomal element‚ it cannot make use of any origin of DNA replication in a chromosome (BP site). Meaning that DNA synthesis within a plasmid depends on having an origin of DNA synthesis of its own. Plasmids are often found in bacterial cells‚ in which they are used as transfer agents for transmitting various antibiotic
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Restriction Endonuclease Digestion of DNA from E. coli cells and Analysis by Agarose Gel Electrophoresis Introduction The main goals of this experiment are testing an alternative procedure called “boiling lysis”‚ evaluating the quality of the purified plasmid for restriction digests‚ and identifying the mislabeled plasmid. The plasmid DNA from a carrier E. coli strain was purified by the boiling lysis. In the boiling lysis method‚ the bacterial cells were given momentary heat treatment
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This lab must be typed. Title DNA Fingerprinting Purpose Why are we doing this lab Background 1. What are restriction enzymes 2. When added to a DNA sample‚ what do restriction enzymes do 3. What do you call the specific sequence of bases the enzyme is searching for 4. What is a restriction digestion 5. What is the purpose of the water bath 6. The electrophoresis apparatus creates an electrical field with positive and negative poles at the ends of the gel. DNA molecules are negatively charged.
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Isolation of plasmid DNA and analysis of isolated plasmid Introduction: A plasmid is an autonomously replicating extra-chromosomal genetic element. In other words‚ this is a DNA molecule external to the bacterial chromosome that is able to replicate on its own and distribute its daughter molecules to daughter cells. You have successfully cloned a fragment of chromosomal DNA containing a tetracycline resistance cassette into a plasmid (pET11a). To this end you have (1) isolated total chromosomal
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Genetic Profiling Genetic profiling is a contemporary issue relating to the individual and technology which restricts access to unbiased decisions and privacy. Genetic profiling interferes with the individuals bodily‚ genetic and behavioural privacy‚ as it can be used for the benefit of identifying bodies to using the results of a DNA test to choose whether to employ one individual over another‚ due to future concerns. It can easily be argued that genetic profiling is in the need of law reform
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Introduction In this lab experiment our group was given four different types of synthetic blood samples‚ along with three types of synthetic anti- A‚ B‚ and Rh‚ to help identify the ABO-Rh blood types. Its purpose of this experiment is to learn about the form and functions of the bloods components‚ as well as to examine and identify different blood types‚ as many already do so and apply it to real life events‚ whether its through forensics‚ crime scene investigations or at hospitals to help identify
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E. Coli Transformation with Plasmid (pGal)‚ pGal Isolation‚ and Analysis of Plasmid DNA Felicia Osadi Bio 22 April 20‚ 2012 Transformation = group 10 Plasmid = group 7 RFLP = group 1 RESULTS Table I. Plasmid Transformation of E. Coli. Plate # | Agar plate | Type | Result | 1 | X-gal | Control | Extensive lawn growth | 2 | Ampr / X-gal | Control | Clear no bacterial growth | 3 | Ampr / X-gal | Transformation | 1 blue colony | Transformation efficiency = 1 transformants
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Ratidzai Lyanne Mapani 1000040207 PRA 0402 Citation Harrison E‚ Koufopanou V‚ Burt A‚ MacLean RC. 2012. The cost of copy number in a selfish genetic element: the 2-μm plasmid of Saccharomyces cerevisiae. J Evol Biol 25(11): 2348-56. What are the actors (e.g.‚ what parts of the organism are in conflict) and what are they in conflict over? The 2 μm plasmid of Saccharomyces yeast is in conflict with the cell host‚ this plasmid cost the host through using the cells’ resources ; meaning
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