“DNA Fingerprinting” Homework Assignment (10 points) Question 1 A schematic of the vector p7012 is shown. The restriction enzymes listed cut only where indicated; they do not cut anywhere else in the vector or insert. a) A schematic of gene W is below. You want to clone all of gene W DNA into the p7012 vector. Give three different strategies that you could use to clone gene W into p7012‚ and obtain colonies that contain a recombinant plasmid. * Strategy 1 uses the restriction enzyme(s)
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Gel Electrophoresis Lab SBI4U1 May 13th‚ 2013 Gel Electrophoresis Lab Purpose: The purpose of this lab is to learn how restriction enzymes cut DNA molecules at specific sequences‚ thus producing DNA fragments of various lengths. Students learn how fragments form unique patterns‚ which help to distinguish the base for DNA identification. This lab answers the question “whose DNA was left behind?”. Materials: * Transfer pipets * Agarose Gel * Dyed DNA samples *
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MEDICAL IMPLICATIONS A genetically modified organism (GMO) is an organism whose genetic material has been altered using genetic engineering techniques. Organisms that have been genetically modified include micro-organisms such as bacteria and yeast‚ insects‚ plants‚ fish‚ and mammals. GMOs are the source of genetically modified foods‚ and are also widely used in scientific research and to produce goods other than food. HUMANS Human gene therapy Gene therapy is the use of DNA as
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Who Killed Rockina? The Crime Late one night‚ the famous rock star‚ Rockina‚ returned to her luxurious apartment from an appearance at a Concert. As she entered her locked apartment‚ she noticed that everything in her apartment was a mess -- the drawers had been emptied out onto the floor; the cushions on the couch were ripped open; and the safe behind the picture on the wall had been opened. She then noticed that the lights were on in her bedroom. She stormed into the bedroom and surprised
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BIO304: Forensic DNA Fingerprinting Lab DNA Fingerprinting‚ method of identification that compares fragments of DNA. It is sometimes called DNA typing. With the exception of identical twins‚ the complete DNA of each individual is unique. A DNA fingerprint is constructed by first extracting a DNA sample from body tissue or fluid such as hair‚ blood‚ or saliva. The sample is then segmented using enzymes‚ and the segments are arranged by size using a process called electrophoresis. The segments are
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Activity 1.1.3 Using DNA to Identify Pathogens Describe the limitations of traditional methods of identifying bacteria. Traditional methods include judging by phenotypic characteristics. This could be a problem if two bacteria looked similar because they could easily be confused. Summarize the goal of each of the six parts of the lab. Sample Prep First we need to extract the DNA from the bacterial wall PCR Amplification Create millions of copies of the initial DNA PCR Purification
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C. partellus gut protease and protease inhibitor activity assays. C. partellus gut protease activity was estimated using chromogenic substrate BApNA [Manasi A et al.‚ 2005]. It was dissolved in the DMSO and a final concentration was made to 1mM in 1.0ml‚ Glycine-NaOH buffer (pH 9)‚ assays were carried at 38°C for 30min [Manasi A et al.‚ 2005]. The reaction was terminated by adding of acetic acid (30%) of 200µl [8‚ 9‚ Manasi A et al.‚ 2005 ]. Supernatant (0.5ml) was added to 1M NaOH (0.5ml) and the
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Zebrafish Embryotoxicity Test (ZET) The ZET will be done following the method proposed by Beekhuijzen et al. (2015) with modifications. Six embryos will be exposed to each of the following treatments: 0.1‚ 0.05‚ 0.025‚ 0.0125‚ and 0.00625 mg/ml. Embryos will be incubated in 24-well plates with built-in lids. Each well will contain one embryo and 2 ml of the respective treatment. All test solutions will be renewed every 24h to maintain the integrity of the test concentrations. The embryos will
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The trial was carried out at the Teaching and Research farm of The College of Education‚ Lanlate to determine the growth characteristics and yield effects of plant spacing and intercropping maize and cowpea. The experimental design used was Randomized Complete Block Design (RCBD) six treatments and five replicates. The treatments were as follows; (1) Cowpea intercropped with maize at 50 X 50cm spacing; (2) Cowpea intercropped with maize at 25 X 25 cm spacing; (3) Sole maize at 50 X 50cm spacing;
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An old well-known method to stabilize a peptide sequences or protein motifs is N-terminal and C-Terminal modification to resistance against exo-nucleases cleavage. Due to its feasibility‚ we decided to perform this type of modification in order to stabilize synthesized sequences based on previous protocols. Understanding the appropriate compound and choosing the way of performance‚ were sensitive and definitely time consuming. Finally‚ N-terminal modification with 5(6) Carboxyfluorescein (FAM) in
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