In this laboratory‚ two experiments were performed; the first one was performed to determine whose fingerprint was found at a crime scene. For this experiment‚ four-10 mL DNA samples were placed in a well of agarose gel‚ using a micropipette; the sample was also covered in a TBE buffer solution. The four samples were then put in the electrophoresis apparatus to be run through electrophoresis. After 20 minutes‚ the samples were ready and the results were documented; it was found that suspect “B”
Premium DNA Fingerprint DNA profiling
Introduction A mutator strain is one that lacks certain repair methods when a mistake is made during DNA replication. The strain of E. coli that was used in this lab‚ XL-1 Red Mutator‚ lacked three different repair methods used to correct mistake in DNA replication. If a mistake occurred‚ the E. coli would not be able to make repairs via DNA polymerase backtracking‚ mismatch repair‚ or oxo-dGTP hydrolysis. The E. coli will still be able to use the other ways to repair mistakes including SOS
Premium Bacteria DNA Escherichia coli
The Effect of Varying Ligation Conditions on Ligation Efficiency Introduction The goal of this experiment was to successfully ligate RFP into P1 and transform E. coli with P1-RFP plasmid‚ which contains the P1 promoter‚ RBS‚ and RFP gene. We tested several different ligation conditions and assessed how they each affected ligation efficiency. The two variables that we tested were: molar ratio of insert (RFP): vector (P1 plasmid)‚ as well as the effect of removing the stuffer from the digested plasmid
Premium Escherichia coli DNA Bacteria
Southern Blotting Southern blotting was named after Edward M. Southern who developed this procedure at Edinburgh University in the 1970s. To oversimplify‚ DNA molecules are transferred from an agarose gel onto a membrane. Southern blotting is designed to locate a particular sequence of DNA within a complex mixture. For example‚ Southern Blotting could be used to locate a particular gene within an entire genome. The amount of DNA needed for this technique is dependent on the size and specific activity
Premium Molecular biology Southern blot Western blot
Genetic Modification of Plants using Agrobacterium Agrobacterium tumefaciens is a rod-shaped aerobic soil bacterium that can infect dicot plants especially apple‚ pear‚ peach‚ cherry‚ almond‚ raspberry‚ rose & grapevines. It’s method of infection is unique‚ for it transfers some of its DNA to the chromosomes of the plant cell‚ causing a tumour to grow & causing the plant to make special organic food molecules for the bacterium. The process begins when a plant is wounded‚ often near the base
Premium DNA Molecular biology Bacteria
Biotechnology industry Analysis BACKGROUND The health care industry Provides health products and service which involved the leading technology of life science. It is a fast-growing industry and will continue to grow in the future because of the increasing request to the advanced medical care. The Global Industry Analysis (GIA‚2009) predicted that the prescription drug market is value around $897 billion in world economy in 2015‚ and Asia-Pacific region is expected to have highest growth in prescription
Premium DNA Gene Genetically modified food
Ethical Issues Associated with Genetic Modification of Plant for Crop Plantation Genetic modification of plant for crop plantation presents to create or maximise desirable characteristic of plant‚ such as increased yield‚ to produce better quality food and resistance to disease or resistance to climatic extremes like heat‚ cold and frost. However‚ most consumers have doubts about genetic modification plants on a less easily defined level. Many consumers could not accept genetic modified plant
Premium Genetically modified food Genetically modified organism DNA
2/15/2013 background on transformation of bacteria with pGLO plasmid Experiment #5 Aim: Purpose of this lab is to have plasmid activity transformed Material: Bacteria starter plate‚ pGLO DNA Plasmid‚ microcentrifuge tubes‚ Ice‚ water bath‚ CaCl2 Transformation solution‚ (LB) agar plate‚ (LB/Amp) agar plate‚ (LB/Amp/ara) agar plate‚ Micropipette‚ and Micropipette tips. Method: Genetic transformation is a procedure which is done by taking genes from one organism and putting them in another organism
Premium Plasmid Gene DNA
Gattaca Assignment In the movie Gattaca‚ three social laws evident throughout the film that relate to present rules in society today include DNA discrimination‚ the assortment of valids and invalids‚ and the exclusion of the disabled. The technique and relation of these futuristic laws verify that just because genetic manipulation is possible‚ does not mean that it should be used in transforming and modifying offspring. In the movie‚ DNA discrimination is apparent from the beginning. The idea
Premium Discrimination DNA Law
In 1989‚ Altman and Cech won a Nobel Prize in Chemistry for their discovery of catalytic RNA – which was instrumental for the RNA World Hypothesis ("Sidney Altman: The RNA World"). This hypothesis stated that early life started with RNA as they possess the ability to serve as a catalyst as well as contain genetic information. The RNA was able to spontaneously form under natural processes‚ which eventually replicated into all sorts of life. This paper will analyze the theoretical and experimental
Premium DNA Gene Genetics