: Joanne Wong Student ID : 00000012636 (BM1/14) Title : Spectrophotometer and its function Introduction Spectrophotometry is the quantitative measurement of the reflection or transmission properties of a material as a function of wavelength.[1] It can measure any of the listed light ranges that usually cover around 200 nm - 2500 nm using different controls and calibrations. [1] There are a few types of spectrophotometer such as calorimeter‚ UV spectrometer‚ IR spectrometer‚ atomic
Free Concentration Solution Light
OBJECTIVES Using spectrophotometric method: determine the wavelengths at which the acid and base forms of the dye in aqueous medium exhibit maximum absorption; determine the molar absorptivities of the acid and base forms of the dye and estimate an unknown concentration of the dye in solution using the Beer-Lambert’s Law; and determine the acid dissociation constant of the indicator dye. THEORY The absorption or reflection of certain wavelengths of light account for observed colors such as the rainbow
Free PH Acid dissociation constant
transmission of material properties as a function of wavelength. It is more specialized than the term electromagnetic spectroscopy which is general in that spectrophotometry deals with near-ultraviolet‚ visible light‚ and near-infrared‚ but doesn’t cover the techniques of time-resolved spectroscopic . Spectrophotometer is used in Spectrophotometry. A spectrophotometer is a photometer that measures the intensity as a function of the wavelength of the light source. Important features of spectrophotometers
Premium Spectroscopy Infrared
referring to electromagnetic radiation that is visible to the human eye‚ and is responsible for the sense of sight.[1] Visible light is usually defined as having a wavelength in the range of 400 nanometres (nm)‚ or 400×10−9 m‚ to 700 nanometres – between the infrared‚ with longer wavelengths and the ultraviolet‚ with shorter wavelengths.[2][3] These numbers do not represent the absolute limits of human vision‚ but the approximate range within which most people can see reasonably well under most circumstances
Premium Light Electromagnetic radiation
objectives for the experiment. 1.)To determine the concentration of a solution of cytochrome c (cyt c) by comparison of its light absorption with the light absorption of a standard 0.1mg/ml solution. 2.)To plot a spectrum of light absorption against wavelength for cyt c. 3.)Prepare a series of cyt c standard solutions of known concentration and to plot a calibration graph of light absorbance against concentration. 4.)Measure the concentration of a solution of cyt c of unknown concentration.
Free Electromagnetic radiation Light Spectroscopy
little equations The electromagnetic spectrum The product of wavelength and frequency is speed is the speed of the wave: Photon Relationship between frequency and photon energy Planck’s Assumption: Photons have momentum but no mass Momentum of photon As wavelength increases of a photon‚ frequency decreases and energy decreases. As wavelength decreases of a photon‚ frequency increases and energy increases. Photoelectric effect Shining
Premium Quantum mechanics Photon Light
The purpose of this lab was to observe the spectral lines of different light sources (Iodine‚ hydrogen‚ helium‚ krypton‚ mercury‚ neon‚ and argon) and to find the wavelength‚ frequency‚ and energy of the emissions of vaporized metallic ions. First‚ we took spectroscopes to look at each light source. The iodine light source seemed coral to the naked eye. When observed with a spectroscope‚ it was clear that there were many red spectral lines followed by relatively similar quantities of orange‚ yellow
Premium
A REPORT ON LASER 1 Techno India College of Technology Megacity‚Rajarhat Kolkata 700156 A Report on LASER TECHNOLOGY By ZAKIR UDDIN AHMAD Student B.Tech 3rd year Applied Electronics and Instrumentation Engineering 2012 2 Abstract The word laser is an acronym that stands for "light amplification by stimulated emission of radiation." In a fairly unsophisticated sense‚ a laser is nothing more than a special flashlight. Energy goes in‚ usually in the form of electricity‚ and light comes out
Premium Laser
Position of Resonance | Second Position of Resonance | Wavelength (m) | Velocity of Sound (m/s) | | 1 | 2 | 3 | Average (cm) | 1 | 2 | 3 | Average (cm) | | | 512 | 15.8 | 15.3 | 15.5 | 15.5 | 49.2 | 49.4 | 49.1 | 49.2 | 0.674 | 345 | 384 | 21.0 | 21.2 | 20.9 | 21.0 | 67.1 | 66.2 | 66.1 | 66.5 | 0.910 | 349 | 320 | 25.2 | 25.4 | 25.1 | 25.2 | 79.4 | 79.5 | 79.7 | 79.5 | 1.090 | 349 | CALCULATIONS Calculation 1: Determining wavelength with frequency. 12λ=L2-L1 12λ512=0.492 m-0.155
Premium Sound
= a() * b * c where A is the measured absorbance‚ a() is a wavelength-dependent absorptivity coefficient‚ b is the path length‚ and c is the analyte concentration. When working in concentration units of molarity‚ the Beer-Lambert law is written as: A = * b * c where is the wavelength-dependent molar absorptivity coefficient with units of M-1 cm-1. Results Table 1: Observations for the maximum absorbance of Fast Green Wavelength Measured Absorbance 460 nm 0.043 480 nm 0.017 500 nm 0
Premium Absorbance Spectroscopy 175