e. Independent: Serial dilutions‚ or % concentrations (unless you are doing Chemistry‚ wherein you could use molar concentrations) could be used to find the optimum‚ and a few samples above the optimum should be done to observe the plateau. f. Control: Just use the same concentration throughout your samples‚ and make note of it in your Design. 4) Enzyme concentration: g. Independent: Depending on the form of the enzyme‚ you could do serial dilutions of digestive enzymes‚
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etc.) Remember to cite information and put responses in your own words to avoid plagiarism (and loss of credit on the assignment). 1. Refer to step 2 in the protocol for lab 6. Calculate the concentration of the sample in tubes 2-5 for the serial dilutions in the chart below. You may wish to refer to http://www.wellesley.edu/Biology/Concepts/Html/standardcurve.html Tube number Water (ml) Blue Solution (ml) Total Final volume (mL) Concentration (mg/ml) 1 0 5.0 2.5 1 mg/ml 2 2.5
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What happens when this enzyme meets up with its substrate? * * * * * 3. Disease samples from two patients are collected and subjected to serial dilutions before running an ELISA. What does it mean if a disease can be detected in samples from one person at a dilution of 1/5 and in another patient at a dilution of 1/100? * * * * 4. Describe a situation that illustrates why it is a good idea to complete the ELISA assay in triplicate. * *
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Effect of Sugar on Bean Plant Growth Abstract The objective: My project was to determine if bean plants grew stronger and healthier by the addition of the right amount of sugar to their watering. I believe that plants that receive 50 grams of sugar per liter of water would help bean plants grow to be stronger‚ healthier and larger because they would get energy from the sugar. Methods/Materials 36 bean plants were grown in potting soil. The same amount of soil was used in each pot and it had no added
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lactose. This makes the area rich enough for the E.coli bacteria to find it easy to adapt to the environment. Some of the methods that were used in part A were streak plating‚ viable plate counting‚ spread plating and serial dilutions. Streak plating involves continuous dilution of the bacteria spread over the surface of the Agar plate using the loop. As the bacteria space out‚ some multiply and form individual colonies over a period of time. When pure coloured strain of colonies are seen‚ it means
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substrate? The enzyme catalyzes the oxidation of the substrate and turns the solution blue. 3. Disease samples from two patients are collected and subjected to serial dilutions before running an ELISA. What does it mean if a disease can be detected in samples from one person at a dilution of 1/5 and in another patient at a dilution of 1/100? One patient has a higher concentration of antigens. 4. Describe a situation that illustrates why it is a good idea to complete the ELISA assay in triplicate
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tested in a spectrometer‚ the highest absorption rates were found to be at 503.7 nm (A=0.281) and 630.7 nm (A=0.270). This lead to the conclusion that the beverage contained red 40 and blue 1. To determine the concentration of the dyes a series of dilutions was prepared for both dyes and tested with the spectrometer. This data was converted into point graphs and a trendline was established. The slope of the trendline (y=mx) was then multiplied by the Absorbance of the associated dye to find the concentration
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Ethanol Production From Food Waste A PROJECT REPORT Submitted in partial fulfillment of the requirements for the Award of the Degree of Bachelor of Technology (Biotechnology) Under the Guidance of Dr. S.M. Bhatt (Associate Professor) Department of Biosciences By Abhishek Agarwal Registration No. 10809065 Roll No. RB18B2A07 Department of Biotechnology Engineering Lovely Professional University Phagwara –144401 November 2011 CERTIFICATE This is to certify that Abhishek
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would turn a detectable form. It catalyzes so the antibody will be easily detachable. 3. Disease samples from two patients are collected and subjected to serial dilutions before running an ELISA. What does it mean if a disease can be detected in samples from one person at a dilution of 1/5 and in another patient at a dilution of 1/100? The one with 1/5 has a more detectable solution and tells scientists that they were one of the first to get it. The one with 1/100 has probably gotten
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mutations work and how they are created is an important step in controlling and isolating genes for experimentation. In this experiment we tested the effects of Ultra-Violet light on growing single-celled yeast. We did this by first creating serial dilutions and growing our yeast colonies with no UV exposure. These yeast cells we used are a Trip1-289 mutant yeast strain and were grown on SC plates with tryptophan present. Next‚ we then grew two more sets of colonies exposed to UV light for pre-determined
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