Serial Dilution Activity Many applications require the determination of microbial numbers. Those applications can be either clinical or in a research setting. Clinical applications include determination of antibiotic efficacy and as well as therapy. Research applications include determination of the effectiveness of antimicrobial chemicals‚ radiation‚ etc. The viable count is most common or standard method used to quantitate bacteria. With this method only microbes that are alive and able to reproduce
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1) Why serial dilutions are preferred to one large dilution in lab experiments? Answer: Serial dilution is the technique of performing repeated dilutions on the same chemical in order to change its concentration. The diluted solution from a serial dilution can be used to calculate the concentration of the actual solution. In experimental work‚ often we need to obtain a range of concentrations for a specific compound. Thus‚ instead of preparing one large dilution‚ if we take a concentrated sample
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1. Introduction: The goal of this lab was to demonstrate the microbiology technique of serial dilutions and how they can effectively be used in experiments. E. coli cells were exposed to UV light for various amounts of time in order to asses the effect on growth and thereby mutations since this cell was modified to not have photolyse no uvr genes for DNA repair and thus can only use the SOS response. Each group then diluted the cells accordingly based on their UV exposure time and counted the cells
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Vocabulary: Lesson pH and dilutions Science Starter: Determine the pH of .0103M H3PO4 solution in water. Remember to look at the H+ count. Box 1 In a dilution‚ the number of solute particles does not change‚ just the concentration with respect to the volume. This relationship is _____________________ proportional. Initially‚ there is 275mL 0.125M stock solution of a substance. -Determine the amount of moles of solute present. -What is the concentration after a dilution when the final volume is
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DILUTIONS AND STANDARDS Many of the laboratory procedures involve the use of dilutions. It is important to understand the concept of dilutions‚ since they are a hand tool used throughout all areas of the clinical laboratory. These dilutions have to be considered as they make a quantitative difference in what is going on. First‚ there are several terms used in expressing dilution: 1. "Dilution: - Dilutions are expressed as the ratio of the quantity of a desired solute (serum‚ urine‚ chemical solution
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CONCENTRATION AND DILUTION Physiology 1‚ Las Positas College Name: In science‚ concentration is a measure of the number of particles (solutes) in a given volume. If one room has 100 people in it‚ and a room of equal size has 50 people‚ one can say that the concentration of people in one room is twice that of the other. Quite simple‚ isn’t it? On a molecular level‚ consider whether you put one lump or two of sugar‚ or no sugar at all‚ in your coffee. If you use two lumps‚ you prefer twice
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A merger is a combination of two companies where one corporation is completely absorbed by another corporation. The less important company loses its identity and becomes part of the more important corporation‚ which retains its identity. It may involve absorption or consolidation. Merger is also defined as amalgamation. Merger is the fusion of two or more existing companies. All assets‚ liabilities and the stock of one company stand transferred to Transferee Company in consideration of payment in
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Preparing for the Future We have all memorized Mr. Nicholson’s annual speech telling us how important it is to focus in school. That focus will certainly help in preparing a competitive admission package as colleges look at many criteria when admitting students. The college application process takes into account your classes taken‚ the level of class difficulty‚ the grade awarded in the class‚ SAT scores‚ and very importantly‚ participation in extracurricular activities. As I listed out the
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Making Molar Solutions and Dilutions Objectives: After performing this lab‚ the student should be able to: • Calculate grams of solute and correctly prepare a molar solution. • Prepare parallel and serial dilutions using C1V1 = C2V2 • Distinguish a parallel dilution from a serial dilution. • Determine whether a parallel or serial dilution should be used in a given situation. • Use a microcentrifuge to make a pellet. Making Molar Solutions and Dilutions A major job of any biotechnician
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No; the experiment was not successful‚ because it did not show the correct fluorescence gradation of the serial dilution of the DNA concentration. As shown by the picture‚ only the first drop of the DNA/EtBr mixture for the DNA standards fluoresce brightly under the UV light‚ while the other spots for both the DNA standards and the unknown DNA standards were all dimly fluoresce. This was due to pipetting error; the tip of the pipette did not touch the liquid (TE) in the micro-centrifuge‚ so no DNA
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