What Is The Fluorestial Dilution Of The DNA Concentration?

No; the experiment was not successful, because it did not show the correct fluorescence gradation of the serial dilution of the DNA concentration. As shown by the picture, only the first drop of the DNA/EtBr mixture for the DNA standards fluoresce brightly under the UV light, while the other spots for both the DNA standards and the unknown DNA standards were all dimly fluoresce. This was due to pipetting error; the tip of the pipette did not touch the liquid (TE) in the micro-centrifuge, so no DNA standards were transferred into the liquid. In the first row (known DNA standards), the DNA was inserted into the first micro-centrifuge, but it was not pipetted from the first to the second micro-centrifuge. In the second row (unknown DNA standards),
(3 points) If the DNA standard is 1 μg/μl, what is the concentration of your unknown sample? Since my EtBr DNA spots did not show a serial dilution, I can not determine the concentration of my unknown DNA sample (#6). However if I did have an accurate set of EtBr DNA spots, I would compare the intensity of each fluorescence drops of the unknown DNA standards to the known DNA standards. The spots that had the same fluorescence would most likely have the same concentrations. If the spots did not have the same fluorescence, then the concentration can be calculated based on the fact that each DNA is five times less concentrated than the prior DNA concentration.

B) Lab 3A: Determine the Concentration of an Unknown DNA Sample by
of Stock μg/μl 1/10 dilution 0.247 0.01235 0.1235 `
1/100 dilution -0.010 -5.00e-4 -0.05

1. (3 points) Which of these OD measurements is more accurate? Why? The 1/10 dilution is more accurate than the 1/100 dilution, because the OD measurement is in the linear range: from 0.05-1. The 1/100 dilution does not fit in the linear range; it goes below the linear range, which indicates that the measurement is not accurate.

C) Comparison of the Spot vs OD Assays

1. (4 points) Compare and comment on the concentration values calculated using the spot method vs the OD method with the spectrophotometer. Which do you think is more accurate? Why?
Although I can not compare the concentration values between the spot method and the OD method, due to the pipetting error in the spot assay, the OD method with the spectrophotometer was more accurate due to the smaller possibilities of error. To add on, the spectrometer is a quantitative research, which means that the data emphasizes an objective measurement with numerical data; it does not include any misinterpretations or