Inspirations Deli Turkey Divided

For experiments 1and 2, many different tests were conducted in order to investigate the different microbes found on the different types of turkey sampled. To begin, our group obtained 5 different forms of turkey that are encountered in everyday life: Inspirations deli turkey from Hannaford supermarkets, turkey bacon, turkey jerky from Whole Foods, Inspirations ground turkey from Hannaford, and a raw whole chunk slice of turkey breast form Whole Foods, these foods were considered experiment 1. Experiment 2 was comprised of deli turkey meat in different forms: Inspirations nitrate-free sliced deli turkey from Hannaford, turkey pre-packaged from a Lunchables lunch packet, Inspirations deli turkey from Hannaford and packaged, sliced, nitrate
To do this, following instructions on page 104 in the Microbiology Lab Manual, molten agarose was poured into a casting tray where it remained for solidification. Once solidified, 300 ml of a buffer was added, this is so the electrical field has something to pass through. Next, each person pipetted 5 microliters from their PCR tubes into the wells inside the gel. Once all the samples were pipetted, the electrical current was added and remained running for anywhere between 10 and 20
To do this, the steps found on page 105 of the Microbiology Lab Manual were conducted without and modifications to the steps. To do this, the remaining 20 microliters was added to a tube containing 100 microliters of CP buffer. Then, the whole sample of both the PCR and CP buffer are added to a blue tube positioned in a white tube. This tube was then centrifuged for one minute forcing and liquid into the white tube and keeping the DNA in the blue tube. Once the centrifuge is done, discard any liquid in the white tube into a waste beaker. Next, 700 microliters of wash duffer was added to the blue column and was then centrifuged for one minute to remove any excess liquid. The wash buffer contained ethanol which DNA is not soluble in meaning it won’t be washed out and will remain in the blue column. Once the centrifuge is finished, the excess liquid was disposed of in the waste beaker. Next, 500 microliters of wash buffer was added to the blue tube which was then centrifuged for one minute to remove the excess liquid which was then discarded in the waste beaker. After applying the wash buffer for the second time, the blue tube was then centrifuged for two minutes to make sure all of the ethanol is out so the DNA isn’t inhibited. The blue column was then transferred to a clean, sterile tube and 50 microliters of elution buffer was added. It’s