One group did not list the identity of their bacteria and one group had listed invalided as their values, so those two groups data were thrown out. As seen above in the graph the positive control shows moderate beta-galactosidase activity and the negative control shows no activity as expected. Looking at the mutant, a large positive trend is seen in Miller Units overtime, more so than the positive control. Thus, the assumption can be confidently made the the mutant can in fact ferment lactose, which aligns with the MAC photos seen earlier. This also means that the mutation itself is likely not in the Lac-Z gene nor the promoter as both would prevent transcription and thereby production of beta-galactosidase. The gene that possess a mutation is likely a missense mutation in the Lac-Y gene or another gene that would still allow for transcription and thus production of beta-galactosidase as seen
One group did not list the identity of their bacteria and one group had listed invalided as their values, so those two groups data were thrown out. As seen above in the graph the positive control shows moderate beta-galactosidase activity and the negative control shows no activity as expected. Looking at the mutant, a large positive trend is seen in Miller Units overtime, more so than the positive control. Thus, the assumption can be confidently made the the mutant can in fact ferment lactose, which aligns with the MAC photos seen earlier. This also means that the mutation itself is likely not in the Lac-Z gene nor the promoter as both would prevent transcription and thereby production of beta-galactosidase. The gene that possess a mutation is likely a missense mutation in the Lac-Y gene or another gene that would still allow for transcription and thus production of beta-galactosidase as seen