Enumeration of Bacterial Contamination in Hamburger Meat from Unknown Sources C March 6‚ 2012 The importance of bacterial enumeration has become even more apparent in recent years due to the increasing numbers of harmful bacteria found in meat products. This process is the key to understanding the populations of microorganisms that contaminate the food supply. Much of the bacteria in meat has been shown to be resistant to multiple drugs; so disease-causing microbes
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experiment allowed the students to perform the plate count technique by serial dilution and two common methods‚ spread plate and pour plate to determine the colony forming unit (CFU) of yeasts A ten-fold dilution is used in this experiment‚ the sample is diluted until it reached the 10-9 dilution. Plating for spread plate started from 10-5‚ 10-6‚ 10-7 and 10-8 dilution while for pour plate‚ it started from 10-6‚ 10-7‚ 10-8 and 10-9 dilution. Having incubated inverted at room temperature (25oC) for 2 days
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What is chromatography- Chromatography is the separation of mixture by passing it in solution or suspension or as vapor. It’s a technique for separating mixtures into the components this needs to happen in order to do the 4 things analyze‚identify‚purify‚quantify. Many scientist use this to do the 4 steps. When analyzing its used to examine the mixture and to find out the relation with one another. In purifying you need to seperate or take away and put it by itself for further study. In identifying
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Student number: 1701974 Unit code: BHS004-1 Assessment number: 2/5 Word count: You were given five samples of patients’ urine and some Uristix® dipstick. Describe how you used the dipsticks to determine if urine sample contained amounts of protein and / or glucose. To test the given samples for glucose or protein‚ dipsticks were immersed into each of the urine samples for 60 seconds. The colour change was compared with the colour chart on the Uristix® bottle. The detection of glucose in urine
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see the effects of pasteurization while emphasizing the process for serial dilutions. PROCEDURE See references (1) RESULTS As the dilution factor increased for both the raw milk (unpasteurized) and pasteurized milk samples‚ the number of colonies decreased. The number of cells/mL in the pasteurized milk sample is considerably less than the number of cells/mL in the raw milk sample. RAW (UNPASTEURIZED) SAMPLE Dilution Factor | Number of Colonies | Number of cells/mL | 10-3 | TMTC |
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growth of yeast is measured by using spectrophotometer and hemocytometer.We learnt how specthophotometer and hemocytometer use and also we learnt qualifications of hemocytometer and spectrophotometer.Serial dilution was used for this experiment and it was very important.Because of the serial dilution‚we measured the number of yeast cells. The graph of growth curve was drawn and bacterial life cycle was understood with the graph.The purpose of the experiment was to calculate and draw bacterial growth
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The latter involves serial dilution and spread plating of bacteria on agar plates. Materials required per pair • One 10 ml liquid culture of Escherichia coli BL21 (see prior preparation) • A sample of Yakult (approximately 5 ml) • Marker pens to label plates & bottles • Sterile plastic loops for streaking bacteria (up to 20 per pair) • Sterile plastic spreaders for spreading bacteria (4 per pair) • Sterile universals/bijoux bottles (8 per pair) for serial dilutions • 10 ml sterile phosphate
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Some food in this world are made using microorganism to produce a desire flavour‚ taste and texture of the food. For examples: yogurt‚ tapai‚ cheese‚ bread and others. Starter cultures is used in these food production. A starter culture is a microbiological culture which actually perform fermentation. These starters usually consist of a cultivation medium‚ such as grains‚ seeds‚ or nutrient liquids that have been well colonized by the microorganisms used for the fermentation. These starters are formed
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is to observe the bacterial growth of Escherichia coli under various conditions. Physical factors and nutritional requirements determine the overall concentration of the bacteria. Along with the use of a spectrophotometer and the technique of serial dilution‚ countable colonies can be obtained. Results are plotted on a semi-log graph in order to observe the different growth curves corresponding to optical density (cell density) vs. time. Materials and Procedure: The materials that were used
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or four minutes for the precipitates to form. Observe the pattern of precipitation. Record. Part II Ksp by dilution of hydroxide ions. 1. Repeat the procedure in Part I‚ but use a serial dilution of NaOH followed by 5 drops of calcium nitrate in each well. Data Analysis Part I 1. Determine the concentration of the calcium ion in each well after the serial dilution but before the sodium hydroxide has been added. 2. Determine the concentration of the calcium ion in each
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