Introduction Bacterial transformation is the permanent alteration of a bacterial cell genotype as a result of its uptake and incorporation of foreign DNA fragments from external medium (Anthony et al‚ 2008). In addition to chromosome‚ bacterial cells often contain extrachromosomal DNA called plasmids which are capable of autonomic replication and antibiotic resistance (Dale & Simon‚ 2010). Plasmids can transport foreign DNA into host or other bacterial cells hence they are known as vectors.
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DCP & CE Title The effect of the different dilutions of yeast cell suspension on the number of yeast cells per cm3 that counted using haemocytometer under microscope. Aim To investigate the effect of the different dilutions of yeast cell suspension on the number of yeast cells per cm3 that counted using haemocytometer under microscope. Research Question Do the different dilutions of yeast cell suspension affect the number of yeast cells per cm3 that counted using haemocytometer under
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Activity 1.1.5: ELISA Introduction Given Sue’s diagnosis‚ all of the patients from the past two days need to be called back in for immediate testing. School officials are concerned about a possible outbreak of bacterial meningitis on campus. In order to diagnose bacterial meningitis‚ it is necessary to obtain a sample of cerebral spinal fluid using a spinal tap. Since this procedure is extremely invasive and painful‚ only those patients doctors feel are at greatest risk for the disease will
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the flask until the meniscus was at the 100 mark. Then I placed the cap on the flask and put it upside down and right-side up‚ mixing it completely. I also had to complete the calculations for the next concentrations using =; I did this for serial dilutions of .0008 mol/‚ .0006 mol/‚ .0004 mol/‚ .0002 mol/‚ and .0001 mol/ concentrations. How many of current concentration necessary for the next desired
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spectrophotometer‚ cuvettes‚ 5mL volumetric pipettes‚ droppers‚ 1mL volumetric pipettes‚ safety goggles‚ rubber gloves‚ and aprons. Procedure Label six test tubes (1 through 5 and B for blank). A series of 5 test solutions of Ferric Chloride are made by serial dilution. Dilute 1Ml of the stock Ferric Chloride to 10 mL with de-ionized water to make a 0.01M solution. Place 1mL of the 0.01M Ferric Chloride solution in the first test tube
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experiment began‚ or was it found based on a similarly known variable so the epsilon value could be known for Beer’s law. Additionally‚ if the epsilon was found from a stock hemoglobin sample what was it’s concentration? Were the dilutions parallel or serial? What dilutions or epsilon value was found?. The experiment can still be conducted effectively without such information‚ but it would have made things easier. Question 2 Outline the procedure you expect to use to determine the pH in your rat’s
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Sterile pipettes and droppers 1) (Phage Serial-Dilution) a) Label broth tubes with dilution factors (1:10‚ 1:100‚ 1:1000‚ 1:10‚000‚ 1:100‚000). b) Pipet 1.mL of stock to the first dilution. Vortex. c) Pipet 1.mL of the 1:10 dilution to the 1:100. Vortex. d) Continue the 10X dilution through to the final concentration. 2) (Phage Plating) e) Label each of the BHI plates according to the various dilutions and one plate “control” which will not include
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An experiment to determine percentage of cell viability by using serial dilutions and a haemocytometer Aim The aim of this experiment was to determine accuracy of the cell count and how valid the result of the experiment will be. Materials and Methods Refer to Scientific and Laboratory skills practical booklet. (Pg. 10-Pg. 12) Results Table one: Raw data (Viable cells) Tube Counts (4x4 grid) Counts (4x4 grid) Counts (4x4 grid) Counts (4x4 grid) Average Count A 34 31
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Retrieved from: http://www.wiley.com/college/spata/samplechapters/ch04.pdf Gopalakrishnan Ilstrup. D. M. (1990).Statistical methods in microbiology http://www.ncbi.nlm.nih.gov/pmc/articles/PMC358156/pdf/cmr00048-0031.pdf Isolation of bacteria by dilution techniques Sharma. D. V.‚ el tol. (2013). Antibacterial and cytotoxic activity of bacillus methylotrophicus-scs2012 isolated from soil. Retrieved from: http://www.jmbfs.org/wp-content/uploads/2013/02/jmbfs_0247_devsharma.pdf Wallenius World Health
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solution at 2 mg/ml in Con A buffer with the hemagglutination reaction of your own purified Con A sample that you diluted previously at 2 mg/ml in Con A buffer. The purpose of this lab was to determine the strength of the reaction by performing serial dilutions on both the Con A sample and the control Con A sample‚ and determine through observations whether or not addition of galactose or mannose will inhibit this reaction. I hypothesize that the Con A + galactose solutions will have partial agglutination
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