Microbiology Coursework: Bacillus cereus After investigation following on outbreak of food poisoning at a pizza restaurant‚ it was found that all suffers had consumed a portion of side salad from the self-service salad bar alongside their main dish. Subsequently‚ this was further traced to a rice salad. Environmental Health Officers investigating this outbreak suspected it may have been caused by Bacillus cereus (B. cereus). The presence of large numbers of B. cereus in a food is indicative
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buffer and Serial dilutions; which you make by diluting the 1% stock solution of amylase into 5 different concentrations (0.5‚ 0.25‚ 0.3125‚ 0.063‚ & 0.0315). Taking the 5 serial dilution tubes‚ you add to each serial dilution tube; 2mL of pH7 buffer‚ 1 mL of 1% starch solution‚ mixing immediately‚ than transfer a drop of the mixed solution into separate wells on the spot plate‚ watching the time to see how long it takes for the color to disappear‚ Repeat this for all the Serial dilutions and record
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Title: Counting the Number of Yeast Cells in a Suspension using Haemocytometer Objective: To estimate the number of cells of yeast per mm3 in five different dilutions of yeast suspension. Introduction: Biologists often need to count the density of cells in a liquid. “Density of cells” means “the number of cells per unit volume of liquid”. For example‚ they might want to find out the density of red blood cells in blood plasma‚ the density of bacteria in milk‚ or the population of Paramecium sp
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Their preference for these sweeteners vary depending on their concentration. To understand the specific concentration of sucrose preferred by Drosophila‚ we exposed them to a serial dilution of sucrose. After obtaining the concentration of sucrose that is most preferred‚ we created a serial dilution of quinine with this concentration to examine whether Drosophila could detect different concentrations of quinine. Since aging in humans is accompanied with a gradual loss of physiological
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protein of influenza viruses to sialic acid. The process requires the saturation of the surface of the virus with Abs. Stable neutralization - with time‚ Ag-Ab complexes usually become more stable (several hours) and the process cannot be reversed by dilution. Neither the virions nor the Abs are permanently changed in stable neutralization‚ for the unchanged components can be recovered. The neutralized virus can be reactivated by proteolytic cleavage. Stable neutralization has a different mechanism to
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determine the effect of enzyme concentration and substrate concentration on the reaction rate‚ we performed a serial dilution of enzymes to get test tubes with full‚ one-half‚ one-fourth‚ and one-eighth concentrations. We then used a spectrophotometer to get the absorbance reading over time‚ and we used these readings to determine the reaction rate. We did the same thing with a serial dilution for substrate concentration‚ and we found the reaction rate for these as well. We found that as enzyme
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Shakira Jarvis Microbiology Lab Assignment Laboratory Assignment Outline 1. Check in & The Microscope a. Review of proper lab etiquette. i. Review laboratory syllabus and b. Review of the Parts of a Microscope ii. Review of lab exercises about different types of Microscopes 2. Survey of Microorganisms c. Viewing‚ drawing‚ and describing several types of fungi‚ algae‚ and Bacteria iii. Chlamydomonas iv. Spirogyra
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in this well‚ 2‚ is now 0.050 M in Ca2 ion.) Use your empty pipet to remove the solution from well 2 and put 5 drops of this solution into well 3. Put the remaining solution back in well 2. Mix the solution in well 3 as before. Continue this serial dilution procedure‚ adding 5 drops of the previous solution to the 5 drops of water in each well down the row until
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Project 1: Antimicrobial Activity of Soil Isolates John Franklin Farrar Department of Biology and Microbiology and Biology Address BOX 22750 BOWEN HALL‚ RALEIGH‚ NC‚ 27607 Abstract: Isolation and characterization of microorganisms is a practice that aids in Increasing ones knowledge of a laboratory setting and it helps improve on Using sterile technique. Isolates of soil microbes can be categorized and Characterized based on a number of criteria ranging from gram-staining
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To make the standard curve was made by serial dilutions. Human control DNA with known concentration was used: 1. First was diluted 1 to 11 and the concentration was 1ug in 5ul 2. 1ul of previous dilution in 9ul H2O > 0.01ug DNA in 5ul 3. 1ul of previous dilution in 9ul H2O > 0.001ug DNA in 5ul 4. 1ul of previous dilution in 9ul H2O > 0.0001ug DNA in 5ul 5. 1ul of previous dilution in 9ul H2O > 0.00001ug DNA in 5ul To make my DNA sample I used dilutions: 1. 1 to 10 2. 1 to 100 The master
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