Antimicrobial Activity of Soil- Research Proposal Abstract

Only available on StudyMode
  • Topic: Bacteria, Microbiology, Antimicrobial
  • Pages : 9 (2797 words )
  • Download(s) : 430
  • Published : May 18, 2013
Open Document
Text Preview
ANTIMICROBIAL ACTIVITY OF SOIL BY SITUMBEKO LIWELEYA (s213459531) Research Proposal Submitted in fulfillment of the requirements for the degree of Bachelor of BIOMEDICAL TECHNOLOGY| |
FACULTY OF HEALTH SCIENCES|
at Nelson Mandela Metropolitan University |
MODULE- RESEARCH METHODOLOGY, SUPERVISOR- PROFESSOR N. SMITH |

Contents
ANTIMICROBIAL PROPERTIES OF SOIL2
ABSTRACT2
LITERATURE REVIEW3
AIM OF RESEARCH4
OBJECTIVES OF STUDY4
RESEARCH METHODOLOGY4
METHOD(S) TO FACILITATE MICROBIAL IDENTIFICATION5
Gram stain5
Culturing5
MATERIALS TO BE USED5
ETHICAL CONSIDERATIONS6
SAMPLE COLLECTION6
SAMPLE ANALYSIS METHOD7
DAY 3 – Expected Results9
DISSEMINATION OF RESULTS9
WORK AND TIME SCHEDULE10
FINANCE BUDGET10
CONCLUSION12
REFERENCE LIST13
BIBLIOGRAPHY14

ANTIMICROBIAL PROPERTIES OF SOIL
ABSTRACT
Isolation and characterization of microorganisms is a practice that aids in increasing ones knowledge of a laboratory setting and it helps improve on using sterile technique. Microbes produce various antibiotics and by isolating different microbes the antibodies can be tested for. Soil samples are highly rich in microorganisms. The antimicrobial activities of microbes from 3 different soil sources (Tenby, Summerstrand, North Campus main building round-about and South Campus water pond) will be tested. Pure colonies isolated from the microbes will be tested for antimicrobial activity against known microorganisms of medical importance; these being E.coli, staphylococcus aureus, Shigella dysenteriae, Shigella sonnei and salmonella spp. Isolates of soil microbes can be categorized and characterized based on a number of criteria ranging from gram-staining which will be done in this project, for enumeration which is a quantitative description based on the amount of microbial colonies available. The results will be recorded. Key words: soil, microbes, microbial activity, antimicrobial resistance INTRODUCTION

It is vital for science today to focus on the isolation and characterisation of an unknown organism expressing interesting properties in relation to antimicrobial Activity. At the conclusion of this laboratory experience the researcher would be required to describe the isolates obtained from the soil macroscopic and microscopic appearance, perform the gram stain of the microorganisms in question and to discriminate the organisms from other microbes that could be contained in the soil and to finally make certain that the organisms have not been exposed to outside sources and be isolated in a pure culture. The researcher must first isolate the microbe then, grow the organism in a pure culture. Pure colonies will be tested for antimicrobial activity against known microorganisms of medical importance. The evaluation of the results that will be achieved are important in the understanding the development of new antimicrobial drugs. LITERATURE REVIEW

Soils typically contain 109 to 1010 microorganisms per gram (dry weight), which may represent more than a million bacterial species. However, characterization of the small fraction of microbes that has been cultivated provides only a glimpse of their potential physiological capacity and influence on soil ecosystems. The absence of pure cultures or genome sequences makes it difficult to ascertain the roles of specific microbes in soil environments. Eichorst, Breznak and Schmidt (2007)

Diarrhoea caused by Shigella species is estimated by WHO to cause 50% of dysentery cases. S. dysenteriae serotype 1 is particularly virulent, causing endemic and epidemic dysentery with high death rate. Salmonella organisms are endemic in many tropical and developing countries, while other salmonellas cause food poisoning and bacteraemia. It is highly infectious and resistance to common available antimicrobials is an increasing problem. Cheesbrough (2000, p. 97)

Antimicrobial resistance is resistance of a microorganism...
tracking img