Pseudomonas Lab Report

The plate count agar, CFC agar and cetrimide agar are used and provided different evidences on the isolation of Pseudomonas from the soil experiment. Firstly, the plate count agar is a medium for the enumeration of viable organisms in food, water, waste water and also from clinical samples, and thus it is non-selective to any species. Whereas the CFC agar is a selective agar which contains reduced amounts of cetrimide but also cephaloridine and fucidin are added to allow the isolation of most Pseudomonas species. If the Pseudomonas species are present, a blue-green or brown pigmentation under the daylight, or fluorescence can be taken as a presumptive evidence under UV light. In the experiment, we had observed the colonies under UV light and
Pseudomonas tends to accumulate in biofilms in filters that are poorly maintained and in areas where pool hydraulics are poor (e.g. under moveable floors). It is also likely that bathers pick up the organisms on their feet and hands and transfer them to the water. It can cause skin, ear and eye infections when present in large numbers and outbreaks of skin infections (especially to people with CF and immunocompromised). The primary health effect associated with the presence of P. aeruginosa is folliculitis, an infection of the hair follicles that may result in a pustular rash. Another health effect is otitis externa, repeated exposure to water is thought to remove the protective wax coating of the external ear canal, predisposing it to infection. Consequently, P. aeruginosa should be monitored at appropriate intervals and thus it is not safe to use the pools if P. aeruginosa is
However, it can also enter our body via different route. If it enters lung tissue, for example in a cystic fibrosis patient, it can cause a form of pneumonia. Patient who being infected may have the following symptoms in different part of the body: pneumonia in the respiratory tract, bacteremia in the bloodstream, diarrhea and enteritis in the GI tract, otitis externa and media in the ear, etc. And unfortunately, the patient in this experiment is likely been infected with P. aeruginosa after tested the sputum sample.

P. aeruginosa infections are considered opportunistic infections, which therefore is a frequent cause of chronic respiratory infection in patients with cystic fibrosis. As many as 80% of cystic fibrosis patients may be colonised in the lung with P. aeruginosa and, once established, it is very resistant to antibiotic treatment.

The increase in antibiotic resistance had made treating infection much more challenging. P. aeruginosa can often develop resistance to multiple types of antibiotics as well as mutate into new, even more resistant forms. However, there are still a number of antibiotic which can tackle P. aeruginosa