The purpose to this lab was to isolate and identify two unknown bacteria from a mixed culture provided to us. This study was done by applying all of the methods that have been instructed on thus far in microbiology laboratory class. Each test performed, provided us with some key information about the unknown microbes in question .
The identification of unknown bacteria is a time honored part of microbiology courses. It will challenge knowledge and skill in performing laboratory techniques, ability to critically evaluate the information obtained from these techniques, and ability to effectively communicate the information.
There are many reasons for knowing the identity of microorganisms. The reasons range from the knowing the causative agent of a disease in a patient, so as to know how it can be treated, to knowing the correct microorganism to be used for making certain antibiotics. This study was done by applying all of the methods that have been learned so far in the microbiology laboratory class for the identification of an unknown bacterium.
a. The unknown is a gram negative bacilli
Upon performing a gram stain on a colony from a streak of the unknown bacteria, at 100x, the bacteria stains negative for the gram stain. In addition, the structure of the bacteria is bacilli.
b. The unknown is a facultative anaerobe
A small amount of bacteria was added to a tube of fluid thioglygollate media, which permits the of a wide variety of bacteria and also allows the determination of the oxygen requirements of an organism.
c. The unknown is nonmotile
The media was inoculated with a single stab to the center of the tube and incubated. After incubation, the growth was restricted to the stab line, the bacteria is nonmotile.
d. The unknown is no capsule formed
The bacteria was removed from a single loopful of broth from the tube and streak it into... [continues]
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