1.1.5 Elisa Testing

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1.,1.5

Activity 1.1.5: ELISA Name _______________________Per_____|

Summarize the steps you took to conduct the ELISA test.

Conclusion
1. Explain why antibodies allow scientists to target and identify specific disease agents. *
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2. Why is the secondary antibody used in an ELISA test conjugated with an enzyme? What happens when this enzyme meets up with its substrate? *
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3. Disease samples from two patients are collected and subjected to serial dilutions before running an ELISA. What does it mean if a disease can be detected in samples from one person at a dilution of 1/5 and in another patient at a dilution of 1/100? *

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4. Describe a situation that illustrates why it is a good idea to complete the ELISA assay in triplicate. *
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5. Why do you think college students living in dorms are often populations who see meningitis outbreaks? *
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6. How did ELISA data allow you to track the path of infection at the college? *
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7. Discuss the limitations of using antigen concentration to deduce the path of infection. Be sure to refer to the workings of the human immune system. *
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8. The ELISA test can also be used to detect antibodies that are produced in response to a specific antigen. Using information about how you completed this ELISA experiment, outline a procedure for testing for antibodies in the blood. *

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9. Explain why in sudden outbreaks, it may be better to test for disease antigens rather than for antibodies. *
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10. Home pregnancy tests utilize ELISA technology. When a woman is pregnant, her body produces a hormone called human chorionic gonadotropin (hCG). Explain how antibodies can be used...
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