Fresh potatoes extract in phosphate buffer pH6 containing the enzyme: polyphenol-oxidase, was used as the variable being tested in this experiment. You may refer to the Enzymatic Reactions Biology 21 Lab Manual. Santa Monica College by Logan, R. 2003 to see how the potatoes extract in phosphate buffer was made. We began this experiment by measuring seven constant amounts of 1ml of 0.1% catechol using a 1 mL pipet into each seven cuvettes. The catechol is our substrate solution. Next, different amounts of phosphate buffer ph.6 of 0.5ml, 1.0ml, 1.5ml, 2.0 ml 2.5ml, 3.0ml and 4.0ml were added to each of the solution of the 0.1% catechol. The purpose of adding phosphate buffer pH6 is to maintain the particular ph6 for the polyphenol-oxidase (enzyme),
As mentioned before, browning in bananas is an enzymatic process where enzymes such as polyphenol oxidase serve as catalysts in the oxidation of several phenolic compounds. In this experiment however, the only reaction being tested is the oxidation of catechol (a diphenol) by the enzyme catalyst catechol oxidase or polyphenol oxidase, which initiates a chain of reactions and eventually triggers the formation of brown pigments known as catechol-melanins.
Catechol, also called 1,2-dihydroxybenzene….
Concentrations of the Enzyme Catechol Oxidase on the Rate of Benzoquinone Production When Mixed with Pure Catechol
November 4th, 2013
Catechol oxidase is an enzyme that speeds up the oxidation reaction when catechol is exposed to oxygen. When the reaction occurs, benzoquinone is produced turning the oxidized substance brown. It was hypothesized that the higher the concentration of catechol oxidase, the browner the substance will turn, and….
Polyphenols act like aspirin in the blood, preventing platelet aggregation or clotting; phenylethylamine acts as a brain stimulant, releasing dopamine which results in a pleasure response of increased awareness and wellbeing, less fatigue and even exhilaration; anandamide-like compounds that block the body's method for destroying naturally occurring anandamide, which keeps it in suspension, able to attach to the CB1 brain receptors that respond to tetrahydro cannabinol, thereby producing a response….
Glucose Oxidase and Its Various Uses
Since glucose oxidase has an end product called hydrogen peroxide, which is a harmful substance to bacteria, it can be used to fight bacteria, or sterilize objects (can have various uses such as in hand sanitizers, toothpaste, soap, etc), not just biosensors.
Another key part in the reaction would be C6H12O6, or glucose. Glucose oxidase can be applied to diabetics as mentioned earlier, as biosensors work by "keeping track of the….
Inhibiting the Action of Catechol Oxidase
By: Kimberly G.
In this lab, Mr. Greene 's sixth period AP Biology class split into groups "to investigate inhibition of enzyme activity by specific chemicals called inhibitors" (1). Group three pondered this lab 's inhibitor, phenylthiourea (PTU).
Is it a competitive inhibitor? That is an inhibitor that literally "competes" with the substrate by mimicking it, and thus "wins" the position at the active site of the enzyme. The blocked….
Microbiology Laboratory Report
Identification of Unknown Bacteria
Authors: Richard Hendricks, Jessica Prebish; NMU
Broth culture 16 was randomly selected by our group and subjected to qualitative tests for taxonomic
identification. The culture did appear homogenous throughout the testing period and is currently retained
by Northern Michigan University’s department of Microbiology. We suggest that culture 16 is an example
of Escherichia coli.
During this unknown lab report various test were performed to differentiate microbes from each other and to compare metabolic and biochemical process.
The gram stain distinguishes between Gram positive and Gram negative bacteria based on the composition of the cell wall. The Gram stain procedure distinguishes between Gram positive and Gram negative groups by coloring these cells red or violet. Gram positive bacteria stain violet due to the presence of a thick layer of peptidoglycan….
Lab Report-Escherichia coli
The purpose for this lab report was to identify and inform of an unknown bacteria that has been causing a patient to have lower abdominal and pelvic pain. To obtain the identification of this unknown bacterium, several biochemical tests needed to be performed in order to prescribe the correct medication to treat and cure the symptoms.
In a lab today, I am to identify an unknown bacterium that is….
In this laboratory exercise explore the differences of microorganism and continue our use of specialized media and use some biochemical testing.This report discloses the basic laboratory instruments will be used in each of our practices .It is of great importance to recognize and identify the different instruments and laboratory tools, because in this way will we be able to use them properly and also to call them by name and know why.
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5cm, 6cm, at the bottom of each test tube.
3. Put your test tubes to the location of the phosphate buffer series and fill each tube according to the following direction.
4. Continue your work area and add 1cm of potato extract obtaining catechol oxidase to each of the seven tubes.
5. Add 1% catechol to each of the 7 test tubes, bringing the total volume to the 6c mark. Agitate the contents of the tubes using a vortex mixer if available.
6. At time 0, record the relative color intensity of each….