Polyphenol Oxidase Lab Report

Fresh potatoes extract in phosphate buffer pH6 containing the enzyme: polyphenol-oxidase, was used as the variable being tested in this experiment. You may refer to the Enzymatic Reactions Biology 21 Lab Manual. Santa Monica College by Logan, R. 2003 to see how the potatoes extract in phosphate buffer was made. We began this experiment by measuring seven constant amounts of 1ml of 0.1% catechol using a 1 mL pipet into each seven cuvettes. The catechol is our substrate solution. Next, different amounts of phosphate buffer ph.6 of 0.5ml, 1.0ml, 1.5ml, 2.0 ml 2.5ml, 3.0ml and 4.0ml were added to each of the solution of the 0.1% catechol. The purpose of adding phosphate buffer pH6 is to maintain the particular ph6 for the polyphenol-oxidase (enzyme),