To determine the effect of various factors on the rate of reaction between an enzyme and its substrate, and also to determine the optimal ranges under which the enzyme activity is maximized. Also to determine whether saline and alcohol are inhibitors or activators
PH factor prediction: I predict that as the pH increases so the activity of the enzyme will increase until it reaches optimum pH range (pH 7) because the enzyme is less denatured when it reaches the preferred pH level, and after this it will decrease because the active site will change in shape and it will no longer accept substrates.
Temperature factor prediction: I predict as the temperature increases, the enzyme activities will increase because there is more energy to speed up the reaction until it reaches the optimum temperature range (room temperature which is about 20 °C), and after that the enzyme activities will decrease because of denature of the enzymes (cause changes to active site that will no longer fit substrate)
Concentration of enzymes prediction: I predict that as the concentration of enzyme increases, so the enzyme activities will increase because there is more enzyme to react with the substrates however when enzymes get saturated, the reaction will come to a plateau because eventually all the substrates will have enzymes to react with, and any extra will have no effect on the reaction whatsoever.
I predict alcohol is an inhibitor of Peroxidase because alcohol when alcohol bind to the allosteric site it changes the active site shape of the enzymes thus deactivating enzymatic activities
I predict salt is an activator of Peroxidase because salt contains Na ions which attaches to the allosteric site changing the shape of the enzyme to fit a substrate.
Materials: • Peroxidase (enzyme in potato) • Hydrogen peroxide, 3% • A strong acid, pH3 (lemon juice, or HCL) 0.5 • A