Enzyme Lab Report
Enzyme Lab Experiments
Problem: How can we demonstrate how enzymes work? What happens if we alter the environment of an enzyme?
Materials:
G;lucose Test Strips Test Tubes Pipettes Raw Hamburg Lettuce Potato
Raw Liver Chalk Beakers Dairy Lactose Tablet Water Sugar
Solo Cups
Hot Plate Knife Gloves Skim Milk Glow Sticks Peroxide
Hypothesis:
1. If we change the environment via temperature the glow stick will
Its intensity will change
2. If hydrogen peroxide is added to a certain food liver then
It would bubble
3. If a lactaid enzyme to milk the
It would separate
Procedure (A):
1. Collect three glow sticks.
2. Boil water in 400 mL beaker add 1st glowstick for 30 minutes.
3. Place second glowstick in freezer for 30 minutes …show more content…
In which beaker did the glow stick glow the most? Why do you think that is?
The beaker that contained hot water. The heat from the water will speed up the chemical rate of reaction taking place, and therefore will glow brighter; the cold one will have its rate of reaction slowed, and thus be dimmer.
2. What did the glow stick show about enzymes and different environments? How does changing the temperature affect the rate of an enzyme-controlled experiment?
The increasing temperature increases molecular motion and may increase the number of times an enzyme contacts and combines with a substrate molecule. Temperature may also influence the shape of the enzyme molecule, making it fit better with the substrate.
3. What effect may change in PH have on an enzyme activity?
The three-dimensional structure of a protein leaves certain side chains exposed. These side chains may attract ions from the environment. Under the right conditions, a group of positively charged hydrogen ions may accumulate on certain parts of an enzyme.
A change in pH disrupts an enzyme's shape and structure. When the pH changes an enzyme's structure, the enzyme can't do its job. Changes in pH break the delicate bonds that maintain an enzyme's shape. An enzyme will unravel, or denature, and become useless in a different …show more content…
A little way down the digestive tract, intestine enzymes need a pH of 8.
4. Why did the hamburger and liver react differently with hydrogen peroxide?
They have natural catalase enzymes which were broken down with the presence of hydrogen peroxide. 5. What is the job of the lactase enzyme?
Lactase is an enzyme which breaks down lactose, a sugar found in milk and other dairy products.
6. In which test tubes was the glucose present? Why?
In test tube #1 the enzyme broke down the lactose . Lactose is a disaccharide of glucose and galactose. The lactose tablet contained lactase which hydrolyzed the lactose into constituent galactose and glucose. Glucose is naturally occurring in skim milk thus in test tube # 2 found in a much smaller amount than test tube #1.
7. What happened when the enzyme was denatured or altered by heat? Did it work the same? Was glucose present?
There was no glucose present. It did not work at all.
Summary: What the class learn today about the jobs of enzymes? What questions does the class still
Problem: How can we demonstrate how enzymes work? What happens if we alter the environment of an enzyme?
Materials:
G;lucose Test Strips Test Tubes Pipettes Raw Hamburg Lettuce Potato
Raw Liver Chalk Beakers Dairy Lactose Tablet Water Sugar
Solo Cups
Hot Plate Knife Gloves Skim Milk Glow Sticks Peroxide
Hypothesis:
1. If we change the environment via temperature the glow stick will
Its intensity will change
2. If hydrogen peroxide is added to a certain food liver then
It would bubble
3. If a lactaid enzyme to milk the
It would separate
Procedure (A):
1. Collect three glow sticks.
2. Boil water in 400 mL beaker add 1st glowstick for 30 minutes.
3. Place second glowstick in freezer for 30 minutes …show more content…
In which beaker did the glow stick glow the most? Why do you think that is?
The beaker that contained hot water. The heat from the water will speed up the chemical rate of reaction taking place, and therefore will glow brighter; the cold one will have its rate of reaction slowed, and thus be dimmer.
2. What did the glow stick show about enzymes and different environments? How does changing the temperature affect the rate of an enzyme-controlled experiment?
The increasing temperature increases molecular motion and may increase the number of times an enzyme contacts and combines with a substrate molecule. Temperature may also influence the shape of the enzyme molecule, making it fit better with the substrate.
3. What effect may change in PH have on an enzyme activity?
The three-dimensional structure of a protein leaves certain side chains exposed. These side chains may attract ions from the environment. Under the right conditions, a group of positively charged hydrogen ions may accumulate on certain parts of an enzyme.
A change in pH disrupts an enzyme's shape and structure. When the pH changes an enzyme's structure, the enzyme can't do its job. Changes in pH break the delicate bonds that maintain an enzyme's shape. An enzyme will unravel, or denature, and become useless in a different …show more content…
A little way down the digestive tract, intestine enzymes need a pH of 8.
4. Why did the hamburger and liver react differently with hydrogen peroxide?
They have natural catalase enzymes which were broken down with the presence of hydrogen peroxide. 5. What is the job of the lactase enzyme?
Lactase is an enzyme which breaks down lactose, a sugar found in milk and other dairy products.
6. In which test tubes was the glucose present? Why?
In test tube #1 the enzyme broke down the lactose . Lactose is a disaccharide of glucose and galactose. The lactose tablet contained lactase which hydrolyzed the lactose into constituent galactose and glucose. Glucose is naturally occurring in skim milk thus in test tube # 2 found in a much smaller amount than test tube #1.
7. What happened when the enzyme was denatured or altered by heat? Did it work the same? Was glucose present?
There was no glucose present. It did not work at all.
Summary: What the class learn today about the jobs of enzymes? What questions does the class still