Peroxidase Lab
TITLE
Analyzing the Effects of pH, Temperature, and Concentration on Peroxidase
ABSTRACT
The purpose of this experiment was to inspect the effect of environmental conditions on hydrogen peroxidase. The three conditions tested were the effect of peroxidase concentration on the rate of the experiment, the effect of pH of the rate of peroxidase activity, and the effect of temperature on the rate of peroxidase activity. During the lab, the lab group tested 7 test tubes, including 1 blank, with different amounts of pH 5 buffer, H2O2, Peroxidase, and Guaiacol. After the certain amount of mL per substance was mixed, the absorbance readings for the effect of peroxidase concentration were taken from the spectrophotometer. The results for the effect …show more content…
A quick and steady hand was important in this step. Make sure to repeat that step for tubes 3-4, 5-6, and 7-8.
RESULTS
Figure 1:
For Figure number 1, the rate of activity steadily increased by approximately .4 each time. From peroxidase concentration 1-2 the rate of peroxidase increased .6 (AU/sec) while the peroxidase concentration from 2 and 3 increased .2. From concentration 1 through 3, the increase in activity was .8 in total.
Figure 2:
For Figure number 2, the increase in activity was .8 from the first and second temperatures. And from the second to third temperature range, the increase was approximately .5. However, from the third to the final temperature, there was a decrease in .8 rate of activity.
Figure 3:
For Figure 3, the rate of peroxidase activity greatly increased from the first to second pH, increasing .7 (AU/sec), while the second to third pH increased only .1. On the other hand, the third to final pH decreased, just like the temperature chart (Figure 2) .45 rate of activity.
Figure 4:
In Figure 4, the mean rate of peroxidase activity increased .004 from the first to the second, while the second to third increased .004 as
Analyzing the Effects of pH, Temperature, and Concentration on Peroxidase
ABSTRACT
The purpose of this experiment was to inspect the effect of environmental conditions on hydrogen peroxidase. The three conditions tested were the effect of peroxidase concentration on the rate of the experiment, the effect of pH of the rate of peroxidase activity, and the effect of temperature on the rate of peroxidase activity. During the lab, the lab group tested 7 test tubes, including 1 blank, with different amounts of pH 5 buffer, H2O2, Peroxidase, and Guaiacol. After the certain amount of mL per substance was mixed, the absorbance readings for the effect of peroxidase concentration were taken from the spectrophotometer. The results for the effect …show more content…
A quick and steady hand was important in this step. Make sure to repeat that step for tubes 3-4, 5-6, and 7-8.
RESULTS
Figure 1:
For Figure number 1, the rate of activity steadily increased by approximately .4 each time. From peroxidase concentration 1-2 the rate of peroxidase increased .6 (AU/sec) while the peroxidase concentration from 2 and 3 increased .2. From concentration 1 through 3, the increase in activity was .8 in total.
Figure 2:
For Figure number 2, the increase in activity was .8 from the first and second temperatures. And from the second to third temperature range, the increase was approximately .5. However, from the third to the final temperature, there was a decrease in .8 rate of activity.
Figure 3:
For Figure 3, the rate of peroxidase activity greatly increased from the first to second pH, increasing .7 (AU/sec), while the second to third pH increased only .1. On the other hand, the third to final pH decreased, just like the temperature chart (Figure 2) .45 rate of activity.
Figure 4:
In Figure 4, the mean rate of peroxidase activity increased .004 from the first to the second, while the second to third increased .004 as