Catalase Lab Report
Enzymes are biological catalysts or assistants that consist of various types of proteins that work to drive the chemical reaction required for a specific action or nutrient. They can either launch a reaction or speed it up. Catalase is a common enzyme found in nearly all living organisms exposed to oxygen. It is a very important enzyme in protecting the cell from oxidative damage by reactive oxygen species (ROS). The catalase used in this experiment will come from five different sources: Spinacia oleracea (Spinach), Brassica oleracea (Broccoli), Solanum tuberosum (Russet Potato), Malus domestica (Apple), and Allium cepa (Onion). The five different catalases from the sources will all be used to catalyze Hydrogen peroxide (H2O2). When hydrogen
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Next, 50ml of distilled water will be measured using a graduated cylinder. The 50g of catalase and the 50ml of distilled water will be put into a blender and blended until liquefied. The catalase solution will then be introduced to a Buchner funnel filtration system to separate the catalase extract from any solid particles left over from the blending process. This step will be repeated until all of the catalases have been blended and filtered. Next, 5 ml of hydrogen peroxide will be measured out using a graduated cylinder. It will then be poured into a small Erlenmeyer flask. This will be repeated five times, representing the five trials for each catalase treatment. Once the Erlenmeyer flask has been given 5ml of hydrogen peroxide, 10ml of the S. oleracea catalase will be measured out and poured into the Hydrogen peroxide filled flask. When the catalase and Hydrogen peroxide meet a reaction will occur and the hydrogen peroxide will be broken down into water and oxygen. The oxygen released from the reaction will be recorded by the Gas Pressure sensor. This step will be repeated five times for each necessary trial, then the next catalase extract will be
Next, 50ml of distilled water will be measured using a graduated cylinder. The 50g of catalase and the 50ml of distilled water will be put into a blender and blended until liquefied. The catalase solution will then be introduced to a Buchner funnel filtration system to separate the catalase extract from any solid particles left over from the blending process. This step will be repeated until all of the catalases have been blended and filtered. Next, 5 ml of hydrogen peroxide will be measured out using a graduated cylinder. It will then be poured into a small Erlenmeyer flask. This will be repeated five times, representing the five trials for each catalase treatment. Once the Erlenmeyer flask has been given 5ml of hydrogen peroxide, 10ml of the S. oleracea catalase will be measured out and poured into the Hydrogen peroxide filled flask. When the catalase and Hydrogen peroxide meet a reaction will occur and the hydrogen peroxide will be broken down into water and oxygen. The oxygen released from the reaction will be recorded by the Gas Pressure sensor. This step will be repeated five times for each necessary trial, then the next catalase extract will be