1) State the specific objective of your study:
2) Why is your study significant, i.e., how is your objective relevant/applicable to the larger world and why would the information you seek be important/valuable to others? (either in terms of using catechol oxidase as a model to study enzyme function or considering the specific activity of catechol oxidase)
3) State your hypothesis (an educated prediction):
Materials and Methods
1) List specific materials used in your experiments (and sources of these materials). Also list any specialized equipment used.
2) In a clear and organized manner, outline your experimental design and describe the procedures you …show more content…
The rate was not affected however. We think this could be because the enzyme had been sitting out for a while and was not working as strongly as it had been before.
4) How do your data fit with what is already known about catechol oxidase? Find as least one research article that is related to your study (either focused on catechol oxidase or a similar parameter tested for a different enzyme), and explain your data in the context of this other study. Cite the article(s) at the bottom of this page.
We found a study about the effects of glycosylation and pH conditions in the dynamics of human arylsuffatase A. They concluded that lowering pH and increasing glycosylation reduced the flexibility of the enzyme. Also, at acidic pH levels the glycosylated enzyme had a higher conformational stability. This study was similar to ours in that it investigated how pH conditions affect enzyme functionality. They found optimal pH conditions for the enzyme like we did, but they took into account another variable that is that influenced functionality