Enzyme Lab Report
Enzymes are proteins that enable chemical reactions. In the enzyme lab, the effects of concentration, temperature and pH on the functionality of the enzyme catalase. The enzyme lab was also about measuring reactions by capturing the oxygen that was generated by the reaction.
Materials and Methods:
Experiment 1, 2, & 3
Experiment 1 examined the effects of concentration on catalase activity.
Experiment 2 examined the effects of concentration in temperature on catalase activity
Experiment 3 examined the effects of pH on catalase activity
The eudiometer was filled with water and inserted into the beaker; the hose was then hooked to the nose of the eudiometer to prevent slipping during the experiment. One of the concentration reactions was put into the flask and after a magnet. The spin was set to 700 rpm, and hydrogen peroxide was injected through a needle into the flask while spinning. Timing began as soon as the hydrogen peroxide was inserted. Once nine milliliters was reached timing stopped. The reaction flask was cleaned after every run.
Materials: Concentrations: 100%, 70%, 50%, & 30%; (1) eudiometer, (1) Large beaker, (1) hose, (1) magnet, (1) flask, spinner, (1) needle containing hydrogen peroxide, and water
Concentration | Time taken to generate 9 ml of Oxygen | 100% | 13.5 seconds | 70% | 16 seconds | 50% | 24.5 seconds | 30% | 39.1 seconds |
Temperature | Time taken to generate 9 ml of Oxygen | Room Celsius | 9.2 seconds | Warm Celsius | 108.4seconds | Hot Celsius | No Reaction |
Concentration | Time taken to generate 9 ml of Oxygen | pH 2 | Enzyme denatured | pH 5 | 17 seconds | pH 7 | 5 seconds | pH 10 | 13.5 seconds | pH 11 | 15 seconds |
Discussion: In table 1 on experiment 1, 100% concentration was the most active and 30% was the least active. The more enzymes-the quicker it attaches the fewer enzymes- the longer it