"Catalase" Essays and Research Papers

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Catalase

Purpose The purpose of this catalase lab is to design simple experiments to demonstrate how various factors affect the rate of enzyme activity. This lab shows how the enzyme decomposes in hydrogen peroxide. Methods and Materials Refer to handout attached to the back of lab Observations Table 1: The mL of oxygen produced with increase of catalase 30secs 60secs 90secs 120secs 150secs 180secs Disks: 2 17ml 16ml 21ml 26ml 31ml 35ml Disks: 4 8ml 19ml 27ml 35ml 44ml 53ml ...

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Catalase Lab

Assessment of Catalase Function Lab Introduction The purpose of this lab report was to test and measure the rate of substrate destruction by an enzyme, we tested the destruction of hydrogen peroxide by the enzyme catalase. Hydrogen peroxide is a poisonous by product of metabolism that can damage cells if it is not removed. Catalase is an enzyme that speeds up the breakdown of hydrogen peroxide into water and oxygen gas. H2O2 + catalase → H2O + O2 A catalyst is a substance that lowers the...

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Potato Catalase Lab

Nicole Mikulis Unit: Biochemistry Sept 14 2012 Lab: Effect of temperature and pH on catalase activity BACKGROUND Catalase is an enzyme that detoxifies chemicals that might harm the cell such as hydrogen peroxide (H2O2). The enzyme breaks H2O2 into water and oxygen. The production of the oxygen gas bubbles serves as evidence that the catalase enzyme is working. As catalase is breaking the bonds between H2O2, it is releasing energy in the form of heat which is reason for...

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The effect of catalase

The Effect of catalase reaction on temperature Table of Contents: Bibliography ………………………………………………………………………………………………….. Page 1 Introduction……………………………………………………………………………………………………Page 2-3 Methods/ Procedures……………………………………………………………………………………..Page 4-5 Results ………………………………………………………………………………………………………….. Page 6 Analysis of Data ……………………………………………………………………………………………. Page 6 Graph …………………………………………………………………………………………………………... Page 7 Conclusion……………………………………………………………………………………………………...

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The Effect of Ph on the Activity of Catalase

Title : THE EFFECT OF PH ON THE ACTIVITY OF CATALASE Aim : To study the effect of pH on the activity of catalase. Introduction : Catalase, an enzyme found in many different tissues, catalyses the breakdown of hydrogen peroxide into water and oxygen. 2 H202 → 2H20 + O2 Hydrogen peroxide is a toxic substance that can be formed during aerobic respiration and catalase removes this product. The activity of catalase can be measured by finding the rate of oxygen release from hydrogen peroxide...

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Identification of Bacteria: Catalase Test

INTRODUCTION The enzyme catalase converts hydrogen peroxide (H2O2) to water and O2. The evolution of O2 causes bubbling. Thus, catalase-positive organisms that are mixed into hydrogen peroxide will cause bubbling (catalase-negative organisms will not). This test is good for distinguishing between Gram-positive cocci in chains (catalase negative) versus Gram-positive cocci in clusters (catalase positive). The coagulase test is used to differentiate Staphylococcus aureus from coagulase-negative...

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Catalase Lab Report

The Catalase Lab Stephen Human Anatomy & Physiology 9/30/12 Problem- How do different environments affect the reactivity of catalase? Hypothesis- If more catalase is added then more oxygen (kPa) will be produced in a faster rate because there is more catalase to react upon. If less catalase is added then less oxygen (kPa) will be produced in a slower rate because there is less catalase to react upon. Variable- Independent- Amount of Catalase (Filter Paper) Dependent- Amount of...

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Effects of Temperature on Catalase Activity.

General Biology I Spring Semester 2013 Lab Report Name: Date: 03/20/2013 Lab: # Purpose of this lab: To test for the effects of temperature on catalase activity. Introduction: Enzymes are proteins that act as catalysts and help complex reactions to occur everywhere in life. Enzymes affect a chemical reaction by acting as a catalyst, which is to say, it speeds up the reaction. However, extreme ph and temperature hinders enzyme function. Optimal activity of an enzyme...

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Effect of temperature on Catalase activity

THE EFFECT OF TEMPERATURE ON CATALASE RESEARCH QUESTION: What is the effect of various temperatures, 0°C, room temperature, 37°C, 50°C, 60°C, on the number of oxygen gas bubbles liberated, in a decomposition reaction between the enzyme Catalase, obtained from crushed mung beans, and 2% of the substrate Hydrogen Peroxide? INTRODUCTION: Enzymes are biological catalysts that increase the rate of chemical reactions without they themselves being involved in the reaction itself. Enzymes are proteins...

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the effect of catalase on hydrogen peroxide

THE EFFECT OF CATALASE ON HYDROGEN PEROXIDE The aim of the experiments is to see if increasing the surface area of the enzyme Catalase, affects the relative activity of the substrate Hydrogen peroxide. Then to observe and measure the effect the Catalase has on the chemical breakdown of the hydrogen peroxide. My theory is if you keep increasing the surface area of Catalase, the more active sites are available to join with the substrate causing an increase in the breakdown of the hydrogen peroxide...

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