Enzymes Lab Report
Lab Report Enzymes (must be typed)
Name: Amanda Gallegos Date: 2/15/15 Section: BIOL 101GL
What is the Hypothesis of this experiment? (3 points)
-The highest temperature will affect the reaction rate of enzymes.
What is the dependent variable? (1 point)
-Change of rate/product/color/absorbency.
What is the independent variable? (1 pt)
-The temperature.
Explain in detail the procedure that you followed (including amount of substrate, enzyme etc, and the whole procedure including incubation times) (3 Points)
-We had three different test tubes. These test tubes were labeled 1, 2, and 3. These tubes all had 3.5 mL water, 2 mL catechol, 0.5 mL extract. Each of these tubes were put into water of three different temperatures for 5 minutes and then were taken to the Spec 20 to check their absorbency. We had a blank tube, which measured 1 mL of potato extract and 5 mL of distilled water, this was how we zeroed the Spec 20.
What are your predicted results? (1 pt)
-I had predicted that tube 3, which was put in boiling water, would have the most changes.
What are the observed results? Draw the table of your results (4 pts)
Tube Numbers
Water Temperature
Absorbency
#1
32⁰C
1.2
#2
2⁰C
0
#3
70⁰C
1.3
What are your conclusions? (Evaluate your Hypothesis) (3pts)
-The colder the water was, the less absorbency each tube had.
Why is this experiment important? (Implications in real life situations- at least 2) (4 pts)
1.) When you have an avocado and leave it at room temperature it will turn brown. When it is brown it is still edible, just usually not preferable. If you have an avocado and put it in the refrigerator, it helps slow down the process of the avocado browning.
2.) When you cut a potato and leave it out for too long they also will turn brown. Let’s say that you want to make mashed potatoes. When you make mashed potatoes, you have to peel the potatoes and then boil them. The heat slows down the breaking down process as well. When you see brown in mashed potatoes it is
Name: Amanda Gallegos Date: 2/15/15 Section: BIOL 101GL
What is the Hypothesis of this experiment? (3 points)
-The highest temperature will affect the reaction rate of enzymes.
What is the dependent variable? (1 point)
-Change of rate/product/color/absorbency.
What is the independent variable? (1 pt)
-The temperature.
Explain in detail the procedure that you followed (including amount of substrate, enzyme etc, and the whole procedure including incubation times) (3 Points)
-We had three different test tubes. These test tubes were labeled 1, 2, and 3. These tubes all had 3.5 mL water, 2 mL catechol, 0.5 mL extract. Each of these tubes were put into water of three different temperatures for 5 minutes and then were taken to the Spec 20 to check their absorbency. We had a blank tube, which measured 1 mL of potato extract and 5 mL of distilled water, this was how we zeroed the Spec 20.
What are your predicted results? (1 pt)
-I had predicted that tube 3, which was put in boiling water, would have the most changes.
What are the observed results? Draw the table of your results (4 pts)
Tube Numbers
Water Temperature
Absorbency
#1
32⁰C
1.2
#2
2⁰C
0
#3
70⁰C
1.3
What are your conclusions? (Evaluate your Hypothesis) (3pts)
-The colder the water was, the less absorbency each tube had.
Why is this experiment important? (Implications in real life situations- at least 2) (4 pts)
1.) When you have an avocado and leave it at room temperature it will turn brown. When it is brown it is still edible, just usually not preferable. If you have an avocado and put it in the refrigerator, it helps slow down the process of the avocado browning.
2.) When you cut a potato and leave it out for too long they also will turn brown. Let’s say that you want to make mashed potatoes. When you make mashed potatoes, you have to peel the potatoes and then boil them. The heat slows down the breaking down process as well. When you see brown in mashed potatoes it is