Enzyme Lab

Topics: Enzyme, PH, Blood / Pages: 5 (1209 words) / Published: Nov 17th, 2011
LABORATORY REPORT
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Activity: Enzyme Activity
Predictions
1. Sucrase will have the greatest activity at pH 6
2. Sucrase will have the greatest activity at
60 °C (140 °F)
3. Sucrase activity decreases with increasing sucrose concentration.
Materials and Methods
Effect of pH on Enzyme Activity.
1. Dependent Variable. amount of product (glucose and fructose) produced
2. Independent Variable. pH 3. Controlled Variables. temperature; amount of substrate (sucrose) present; sucrase + sucrose incubation time
Effect of Temperature on Enzyme Activity.
1. Dependent Variable. amount of product (glucose and fructose) produced
2. Independent Variable. temperature 3. Controlled Variables. pH; amount of substrate (sucrose) present; sucrase + sucrose incubation time
Effect of Substrate Concentration on Enzyme Activity.
1. Dependent Variable. amount of product (glucose and fructose) produced
2. Independent Variable. amount of substrate (sucrose) present
3. Controlled Variables. temperature; pH; sucrase + sucrose incubation time
1. Describe what is measured as an indicator of sucrase activity and why this is an indicator of sucrase activity.
Amount of product (glucose and fructose) produced is what is being measured for this lab. This is an indicator of sucrase activity because the amount of product produced is a result of the expiriment itself.
2. Explain why denatured sucrase was used as a control.
Denatured sucrase was used as a control because activated sucrase would increase the activity.
3. Alkaline DNS denatures sucrase. Explain why it is important to denature the enzyme.
It is important to denature the enzyme because leaving it active would increase the activity of the enzyme.
Results
Table 1: Effect of pH on Sucrase Activity
Optical Density ph 3 ph 4 ph 5 ph 6 ph 7 ph 8 ph 9
1 0.156 0.292 0.653 0.935 0.782 0.429 0.151
2 0.165 0.321 0.666 0.92 0.763 0.429 0.151

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