Biology 101 Lab Paper

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Biology 101 Lab Paper
Objective of experiment * The objective of the experiment is to assess the effect of different pH conditions on enzyme activity.
Hypothesis
* I feel the pH affected the enzyme activity to produce product.
Prediction
* If the pH affected the enzyme activity to produce product, then rate of reaction will change.
Procedure
* The procedure that we followed was to take two test tubes and label one pH2 and one pH10 and incubate 1 ml of each solution for 30 minutes. Then after 30 minutes we added a) 3.5 ml buffer b) 0.5 ml ONPG (substrate) c) 0.5 ml of the dilution that was incubated with the buffer (enzyme)
Then we allowed the reactions to proceed for the 19 seconds, which we used in activity 2 for the pH7. Then we stopped it with 0.5 ml sodium carbonate. Then put it in the sec 20 to determine the absorbance of each solution. The concentration enzyme was obtained by with the standard curve as attached. The rate of reaction is calculated by concentration/time. * The dependent variable is the rate of reaction. The independent variable is pH levels. The controlled variable is temperature in the room and the enzyme concentration. Enzyme pHLevel | Absorbance | ProductConcentration | Rate | pH7 | 1.134 | 312.89 | 1043 nmol/min | pH2 | 0.491 | 134.28 | 447.6 nmol/min | pH10 | 0.105 | 27.05 | 90.17 nmol/min |

Conclusion * The conclusion of this experiment was supported by the rate. The results between the pH 2 and Ph10 decreased compared to the pH 7 found in activity #2. The absorbance found is that it does procedure product. My results agree with my

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