Easy Peasy
Prac 3
The effect of change substrate concentration on the activity of an enzyme.
Purpose
To investigate the effect of change on the substrate concentration on the activity of an enzyme(catalase).
Materials
Potato, mortar & pestle, 4 test tubes, test tube rack, spatula, hydrogen peroxide 6%, 3%, 1.5%, pipettes, scalpel, ruler.
Procedure 1. Cut using scalpel several raw potato cubes each measuring 1 cubic centimeter (cc). 2. Each cube was ground with a mortar & pestle and then added to a test tube using a spatula. 3. Test tube 1 was left as the control with no added hydrogen peroxide. 4. Different concentrations of hydrogen peroxide solution were added to each test tube using a pipette. (5mL of 6%, 3% and 1.5%) 5. The rate of reaction was observed. (the rate of bubble production.
Risk assessment * Ensure gloves are worn to prevent burning of the skin by hydrogen peroxide.
Results
3% created the most amount of bubbles, 6% creating the second most and 1.5% creating the least.
Discussion 1. Which solution of H202 had the fastest rate of bubble production and which one had the slowest rate.
6% of H2O2 was meant to be the fastest and 1.5% the slowest. 2. What gas was released?
Oxygen
3. Why did you ground the potato with mortar & pestle?
To increase the surface area/volume ration. The potato was ground as catalase is an intracellular enzyme and cell walls and membranes need to be broken down to release the enzyme. 4. What was the source of enzyme catalase in this experiment?
Potato
5. What difficulties did you encounter when doing this practical?
It was difficult to ensure that the same amount of potato was added to each test tube. Hard to add solution of H2O2 at the same time to see reliable results. 6. Draw a graph to show how the rate of reaction changes with the change in substrate concentration. 7. Why was a control used?
To use as a comparison to the rest of the samples.
Conclusion
The more concentrated the
The effect of change substrate concentration on the activity of an enzyme.
Purpose
To investigate the effect of change on the substrate concentration on the activity of an enzyme(catalase).
Materials
Potato, mortar & pestle, 4 test tubes, test tube rack, spatula, hydrogen peroxide 6%, 3%, 1.5%, pipettes, scalpel, ruler.
Procedure 1. Cut using scalpel several raw potato cubes each measuring 1 cubic centimeter (cc). 2. Each cube was ground with a mortar & pestle and then added to a test tube using a spatula. 3. Test tube 1 was left as the control with no added hydrogen peroxide. 4. Different concentrations of hydrogen peroxide solution were added to each test tube using a pipette. (5mL of 6%, 3% and 1.5%) 5. The rate of reaction was observed. (the rate of bubble production.
Risk assessment * Ensure gloves are worn to prevent burning of the skin by hydrogen peroxide.
Results
3% created the most amount of bubbles, 6% creating the second most and 1.5% creating the least.
Discussion 1. Which solution of H202 had the fastest rate of bubble production and which one had the slowest rate.
6% of H2O2 was meant to be the fastest and 1.5% the slowest. 2. What gas was released?
Oxygen
3. Why did you ground the potato with mortar & pestle?
To increase the surface area/volume ration. The potato was ground as catalase is an intracellular enzyme and cell walls and membranes need to be broken down to release the enzyme. 4. What was the source of enzyme catalase in this experiment?
Potato
5. What difficulties did you encounter when doing this practical?
It was difficult to ensure that the same amount of potato was added to each test tube. Hard to add solution of H2O2 at the same time to see reliable results. 6. Draw a graph to show how the rate of reaction changes with the change in substrate concentration. 7. Why was a control used?
To use as a comparison to the rest of the samples.
Conclusion
The more concentrated the