Extraction of Caffeine from Tea Leaves Introduction Caffeine is soluble in boiling water and as a result it is easily extracted from tea bags by steeping in hot water. This process leaves behind the water insoluble portions of the tea bag. However‚ water extracts more than just caffeine‚ so a final separation is done with an organic solvent that will dissolve primarily caffeine. The organic solvent used in this experiment is Dichloromethane (CH₂Cl₂). Dichloromethane is less polar than water
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Extraction and Sublimation of Caffeine from Tea Leaves By: Ashley Barnes CH 236 – P8 Experiment 4 & 4.5 Due: 10/15/2014 Table 1. Table of Reagents Discussion The extraction yield refers to the percent of caffeine in the tea leaves at start of the extraction experiment. Tea leaves had 0.55 grams of mass per eight ounce serving. The weight of the extracted crude caffeine was 0.264 grams. Therefore‚ the extraction yield was 48%. This means that we pulled out almost half of the caffeine
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Summary Caffeine is a stimulant for the central nervous system which will relax the muscle and increase the gastric acid and heart rate (Kelly‚ 2017). The analyzing of caffeine and caffeine metabolites in saliva is to quantitatively measure the ratio of concentrations of caffeine and metabolites. There are three different metabolites of caffeine and the majority of caffeine metabolite is paraxanthine. The ratio of concentration represents the activity of the first metabolism enzyme of caffeine‚ CYP1A2
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Isolation of Caffeine from Tea (12A) Introduction When a person drinks tea or coffee‚ it is not pure caffeine. It also has other natural substances. The purpose of this lab was to isolate caffeine from tea leaves. The methylene chloride removed nearly pure caffeine from the basic tea solution. Caffeine is a white‚ crystalline‚ bitter alkaloid‚ C8H10N4O2 ‚ usually derived from coffee or tea. Caffeine is used in medicine chiefly as a nervous system stimulant. Caffeine is used by humans to ward off
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E5 & E6 Extraction of Chlorophyll from Fresh Spinach and Investigation of the Photochemistry of Chlorophyll Chlorophyll a Chlorophyll b E5 - Extraction of Chlorophyll from Fresh Spinach E6 - Investigation of the Photochemistry of Chlorophyll The aim of this experiment is to investigate the photochemistry of chlorophyll. This experiment will be performed in two lab periods. In the first lab period you will extract chlorophyll‚ the green pigment in leaves‚ from spinach.
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Biotechnology Lab Report Lab: Extracting DNA from Bananas and Strawberries Purpose: To properly and successfully extract DNA from various fruits using cell disruption and separation techniques. Materials Used: 2 heavy duty zip-lock baggie 1 strawberry (fresh or frozen and thawed) 1 banana half 10 ml DNA extraction buffer* 2 Coffee filters Ice cold 95% ethanol 1 small beaker 2 Test tubes Wooden coffee stirrer *To make the extraction buffer‚ 100 ml of shampoo (without conditioner) was mixed
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Result of Extraction Weight (g) Tea Leaves 5.5783 Crude Caffeine 0.0246 Empty Beaker 117.5350 Beaker + Crude Caffeine 117.5596 The table above shows the weight of the sample tea leaves‚ the crude caffeine content in the sample‚ the empty beaker used in the extraction‚ and the beaker with the crude caffeine. It is induced from there that for that amount of sample tea leaves‚ which is equivalent to three bags of Nature’s Pride Tea; there is a 0.0246 g content of crude caffeine for every
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Kadijat‚ and Kharla Ms. Bulahan Writer: Djina Jan-18-15 Lab #6 - DNA Extraction lab Introduction: DNA is a double stranded macromolecule composed of nucleotide bases pairing Adenine with Thymine and Cytosine with Guanine. S ince DNA is the blueprint for life‚ every living thing contains DNA. The extraction of DNA from cells and its purification are of primary importance to the field of biotechnology and forensics. Extraction and purification of DNA are the first steps in the analysis
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reaction mechanism since no chemical identity had been changed. The lab demonstrates the use of chemical molecular behavior to isolate a particular set of molecules. Caffeine had already existed in the leaf itself but needed to be separated from the other chemicals. Caffeine’s chemical structure is relatively similar to the nucleic acid purine in that they use nitrogen and is bicyclic but lacks an alkene‚ amine and an amide. Caffeine has a solubility of 67.0 g/100 mL in boiling water but tannins also
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MULTIPLE EXTRACTION OF CAFFEINE FROM DRIED TEA LEAVES USING DICHLOROMETHANE Delos Reyes‚ K.‚ Dizon‚ G.J.‚ Enriquez‚ J.R.‚ Estrada‚ G. and Garcia C. Group 4 2G Med Tech Organic Chemistry Laboratory ABSTRACT Caffeine was extracted from dried tea leaves by multiple extraction technique. 10g of tea leaves was boiled in a solution of 4.4 g anhydrous sodium carbonate and 100ml distilled water and was extracted three times using 20 ml of dichloromethane. Theresidue was collected by decanting
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