This experiment involves an extraction of a natural product using the techniques of steam distillation. The principle component of oil of cloves is an aromatic compound‚ which is identified by thin layer chromatography (TLC). Eugenol is widely used in dentistry‚ due to its analgesic‚ antiseptic balsamic qualities. It is ideal for curative for pulp hyperemia (the soft‚ sensitive substance underneath the enamel and dentine of a tooth) and pulp its. In dentistry eugenol is also used to cover cavities
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In this experiment‚ the essential oil eugenol was isolated from ground cloves using steam distillation techniques. Eugenol had a high boiling point‚ but it was isolated at a lower temperature by performing a co-distillation with water‚ a process known as steam distillation. This technique allowed the desired material to be distilled at a lower temperature than 100℃. The point of the steam distillation was to use an azeotrope to isolate the organic compound from a solid. The azeotrope was the liquid
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Isolation of Acetyleugenol (Clove Oil) from Cloves Purpose In this experiment‚ you will obtain the essential oil from cloves by steam distillation. You will then separate a component (acetyleugenol) from the oil by liquid-liquid extraction. Finally‚ you will isolate the acetyleugenol by using a rotovap for reduced pressure distillation‚ which will remove the organic solvent (dichloromethane). Background Whole cloves contain between 14% and 20% by weight of essential oils‚ but
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Steam distillation of cloves produced 0.53g of an oil which contained in its IR spectrum the functional groups O-H (at 3520 cm-1)‚ sp2 C-H (3080 – 3000 cm-1)‚ aliphatic C-H (2980 – 2940 cm-1)‚ and both alkene C=C (at 1640 cm-1) and aromatic C=C (at 1514 cm-1). The IR spectrum is attached to this report. These data are consistent with the structure of eugenol‚ shown in Figure 2 below: In addition‚ the IR of the product from the steam distillation of cloves closely corresponds with that of an
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Isolation of Eugenol from Cloves by Steam Distillation and its Identification by Infrared Spectroscopy Eim A. Chemist CHEM 303 June 16‚ 2005 INTRODUCTION “Essential oils” are the volatile components associated with the aromas of many plants.1 In this experiment‚ the essential oil eugenol (the main component of oil of cloves) will be isolated from ground cloves using the technique of steam distillation‚ which is often used to isolate liquid natural products from plants.2 The principle
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Steam Distillation – Clove Oil Abstract: In this experiment‚ a situ method steam distillation was performed and essential oils were isolated from cloves. Once the oils were obtained‚ extraction techniques were used to extract a crude‚ eugenol‚ and acetyleugenol product sample. These samples were submitted for GC analysis and the normalization area percents were calculated to confirm their purity; for the crude sample it was 93.95% eugenol and 6.05% acetyleugenol‚ for the eugenol sample it
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Edward Cross Organic Chemistry December 10‚ 2012 Title Steam Distillation of Cloves and Extraction of Eugenol Authors Edward J. Cross* Summary The isolation of Eugenol from Cloves begins with whole cloves and then using a steam distillation apparatus to obtain the cloves oil substance. Then with the mixtures of water and dichloromethane to form two immiscible layers. The layers were dichloromethane substance and aqueous The entire substances that are separated into two individual
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Isolation of Eugenol from Cloves Abstract The experiment conducted‚ isolation of eugenol from cloves‚ is the extraction of the essential oil. To perform this experiment cloves and water were mixed together and heated by a heating mantle in a round-bottom flask. A direct stem distillation took place‚ and the oil product was out aside for a week. After one week‚ the isolation of the eugenol actually takes place. Next the thin-layer chromatographic analysis takes place‚ but the experiment
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Extraction of Eugenol from Cloves 02/01/2014 CHM2210L University of North Florida Abstract Eugenol is found in the essential oil of cloves and has distinct properties that make it an important product to both food and drug industries. (Bhimrao‚ et. al‚ 2004). To utilize many of eugenol’s characteristic properties‚ it is necessary to isolate this organic chemical from the other components of cloves. In this experiment‚ eugenol was isolated from a sample of cloves using a series of techniques
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2: Isolation of Eugenol from Cloves Background; Readings on Vapor pressure‚ Raoult’s Law from TRO: A mixture of the essential oils‚ eugenol and acetyleugenol‚ will be steam distilled from cloves. These compounds are isolated from aqueous distillate by extraction into dichloromethane. The dichloromethane solution is shaken with aqueous sodium hydroxide‚ which will react with eugenol‚ to yield the sodium salt of eugenol in the basic aqueous layer
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