Title of Experiment Extraction of Spinach Date that the Experiment was Performed This experiment was performed on Wednesday‚ September 17th‚ 2014 at 2:45 pm in the St Ignatius Science Center Laboratory 323. Partners Names Taylor Jackson and Matt D’Angelo. Taylor‚ Matt‚ and I shared the same data. Purpose/Goals/Objectives The purpose of this experiment was for each student to use column chromatography to separate plant pigments from spinach leaves. Some goals and objectives were to
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take spinach leaves and extract the chlorophyll and carotenoid pigments by using acetone as the solvent. The chlorophyll and carotenoid pigments were extracted by using column chromography and alumina was used as the solvent. Solvents of different polarities were used‚ starting with the least polar‚ to extract the certain components from the leaves. They were then analyzed by using thin- layer chromatography. Procedure: The first part of the experiment dealt with breaking down the spinach leaves
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& E6 Extraction of Chlorophyll from Fresh Spinach and Investigation of the Photochemistry of Chlorophyll Chlorophyll a Chlorophyll b E5 - Extraction of Chlorophyll from Fresh Spinach E6 - Investigation of the Photochemistry of Chlorophyll The aim of this experiment is to investigate the photochemistry of chlorophyll. This experiment will be performed in two lab periods. In the first lab period you will extract chlorophyll‚ the green pigment in leaves‚ from spinach. In the
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Absorption spectrum of the Spinach Chloroplast By a Different Wavelength and Photosynthesizes Activity in different light filters Instructor: Dr‚ David Danseraveu Student: Hassan Sedaghat‚ A00345910 Due date: 11/13/2012 Material and Method: In the photosynthesis system‚ plants and other photosynthesizing creatures they use light of the sun as energy by absorbing some specific wavelength. A prepared sample of the chloroplast from spinach was used during this experiment
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three smaller experiments as a result of the different properties chlorophylls possess and the need to test them individually to receive the most accurate results. Preparation for the lab began with selecting a medium to large spinach leaf and deveining it. Once the spinach was prepared‚ it was ground in 5 mL of 100% acetone‚ creating a green acetone extract. The extract was filtered through a funnel with a kimwipe and into a test tube. The volume of the filtered extract was estimated‚ and one half
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results did not support the hypothesis. Referring to table‚ one can see that blue caused both the spinach and radish plant to grow the most and that green light cause the plants to grow less. Spinach is a long-day plant therefore it was able to grow better under a constant light source. This would have aided in the plant growing faster than other forms of plant species. When experimenting with the spinach plant blue light worked exceedingly well in making the plant grow faster with there being a decent
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the Extraction lab is to separate and purify benzoic acid‚ 2-naphthol‚ and naphthalene. These organic solids are purified by partitioning the solid in 2 immiscible solvents‚ diethyl ether and sodium bicarbonate or sodium hydroxide. II. Significance a. The significance of the Extraction lab is to purify Acetanilide benzoic acid‚ 2-naphthol‚ and naphthalene by determining the partition coefficient. This value is determined by dividing the solubility of the given solute in the extraction solvent
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CH 223 - Organic Chemistry Laboratory I Experiment #6 - Column and Thin-Layer Chromatography (TLC) of a Spinach Extract Pre-Lab Assignment (1) List 5 ways that TLC can be used in an organic chemistry lab experiment. To identify an unknown‚ to monitor the course of a reaction and assess the purity of its product‚ to determine the best solvent for a column chromatography separation‚ to determine the somposition of each fraction from a column chromatography separation‚ and to determine whether
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Extraction Separation of a Carboxylic Acid‚ a Phenol‚ and a Neutral Substance Purpose The objective of the experiment is to identify the three substances in the mixture and to determine the percent recovery of each from the mixture. The unknown will consist of either benzoic acid or 2-chlorobenzoic acid‚ 4-t-butyl phenol. Determine the weight of crystals and also the percent yield Introduction There are various techniques of extractions‚ solid/liquid extraction‚ which
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wallet which means there’s spare cash for those much wanted days out. Take a look at our top 3 must haves for your garden this December. 1. Spinach Spinach is a great way to add iron to anyone’s diet whether you are pregnant‚ suffering iron deficiency or simply want to feel like Pop-eye this veggie gives a fantastic natural boost. For best results‚ spinach seeds should be planted in well drained soil. Place the seeds between ½ an inch and an inch apart‚ 1 inch into the soil and lightly cover‚ they
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