EXTRACTION AND CHARACTERIZATION OF PROTEINS Abstract Different techniques and principles for protein extraction and characterization were demonstrated in this experiment. Various proteins were extracted from different sources: 1.67 g yeast invertase‚ 1.03 g egg white albumin‚ and 5.15 g of milk casein. Activity assay for invertase was performed using Benedict’s test and the enzymes inverting action on sucrose was confirmed. Warburg-Christian Method and Bradford Assay were also employed to determine
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Lab 4: Photosynthesis and Chromatography of Spinach Leaves Discussion: The chromatography of the spinach leaves was taken to establish what pigments were present in the chloroplasts of the leaves. The solvent carried and separated the different pigments up the cellulose paper. The solvent moved up the paper because of capillary action because of adhesion of the solvent molecules and the paper. The pigments dissolved in the solvent also were carried up the paper and were separated because
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Surgical extraction I start by escorting the patient into the surgery I put full personal protective equipment on myself and the patient‚ I have placed all needed instruments on the work top for the dentist to access. Instruments: * Mirror‚ probe and tweezers * Local anaesthetic equipment * Aspirator and saliva ejector * Hand piece (fast slow and straight) and surgical burs * Scapel‚ periosteal elevator and swabs * Retractors * Forceps * Elevators * Bone forceps
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coffee. The average pure caffeine yield was 0.330% with a standard deviation of 0.179. This yield is slightly higher than the yield of this report because of the error that occurred during the experiment. A small amount of coffee from the first extraction was stuck in the nozzle of the separatory funnel so it was removed with a pipet and then decanted. This resulted in a loss of product. The average impure caffeine yield was found to be .948% with a standard
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TWO-BASE EXTRACTION OF BENZOIC ACID‚ 2-NAPHTHOL‚ AND NAPHTHALENE FROM UNKNOWN SAMPLE # 131 Douglas G. Balmer (T.A. Mike Hall) Dr. Dailey Submitted 11 July 2007 Introduction: The purpose of this experiment was to separate a sample of benzoic acid‚ 2- naphthol‚ and naphthalene of unknown proportions using a two-base extraction method. The three components of the mixture will react differently to sodium bicarbonate and sodium hydroxide because each of the bases’ conjugate acids has a different
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Title Page Chelsea Maksin Organic Chemistry Laboratory 1 Column and Thin Layer Chromatography: The Separation of Spinach Pigments Russ Hoburg 02/15/2012 Abstract The main goal in doing the column and thin layer chromatography was to separate spinach extract into its components based on polarity and then to analyze the components. The separation of the spinach extract was done using the column chromatography with the wet/slurry packing method. Alumina was inserted into the column to act as
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The Cell Extraction In the dangerous occupation of a correctional professional the “cell extraction” is something that will happen. People can get angry if they don’t get their way. I say people because inmates are people‚ that got into trouble. As a correctional professional I can use the “cell extraction” to move an incompliant inmate from one cell to a more secured cell. This is something I am good at. I have to be. It is my job. The first time that I required an extraction team to suit up
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Isolation of plasmid DNA and analysis of isolated plasmid Introduction: A plasmid is an autonomously replicating extra-chromosomal genetic element. In other words‚ this is a DNA molecule external to the bacterial chromosome that is able to replicate on its own and distribute its daughter molecules to daughter cells. You have successfully cloned a fragment of chromosomal DNA containing a tetracycline resistance cassette into a plasmid (pET11a). To this end you have (1) isolated total chromosomal
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Objective: The purpose of this lab is to allow you to become familiar with a procedure for extracting DNA‚ collecting DNA samples‚ and to observe the physical characteristics of DNA Background: The wild strawberry is a diploid because it only has two sets of chromosomes. While the grocery store kind is an octoploidy because it has eight sets of chromosomes. The reason we chose the grocery store strawberry is because we’d be able to extract more DNA. Ripe strawberries make enzymes which help break
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patient. However‚ we are only interested in the patient’s head and more specifically the brain‚ that is why in this step we perform an orientation step to orient all the brain images into a common coordination and then we perform the brain extraction using brain extraction tool (BET) which will result two images‚ one image contains only the brain and the other one contains the brain and the skull‚ both images are needed for further analysis‚ Figure 2.4 and Figure 2.5 Show the MRI image before and after
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