Liquid CO2 Extraction of D-Limonene This convenient‚ effective and inexpensive liquid CO2-based extraction of a natural product (D-limonene) provides an engaging example of natural products extraction using an alternative solvent (liquefied CO2). Because the procedure can be carried out in a polypropylene centrifuge tube and uses crushed dry ice as its CO2 source‚ it provides an inexpensive alternative to the equipment-intensive extraction with supercritical CO2. A short video clip is available
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Extraction of Caffeine from Tea Leaves using Dichloromethane (CH2Cl2) Abstract The extraction of caffeine (C8H10N402) was done in two parts. The first procedure used was solid-liquid extraction which was done by boiling the tea leaves in 100 ml. distilled water for 5-10 minutes. The filtrate was acquired and was extracted again by liquid-liquid extraction using dichloromethane (CH2Cl2). The combined DCM (CH2Cl2) was washed and dried to yield crude caffeine. The crude caffeine was purified
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Introduction Extraction involves dissolving a compound or compounds either from a solid into a solvent or from a solution into another solvent. Extraction is a method used to purify a substance by removing the impurities that was added to it. A solvent- solvent extraction is a type of extraction that is most commonly done by using two substances that are typically insoluble with each other. An acid-base extraction is a solvent-solvent extraction‚ in almost every case one of the solvents is water
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Extraction Report For experience 4c we were supposed to determine which one is the organic layer in three different tubes that has two layers. To determine which is aqueous (water) or organic‚ I used the technique of adding droplets of water to each tube. If the layer is water‚ then the drops of added water will dissolve in the aqueous layer and increase it’s volume. If the added water form droplets or a new layer‚ then it is the organic layer. Tube 1 (water and n-butyl chloride)‚ after adding
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Preparation of SKEO The extraction of S. khuzistanica was performed by hydro distillation and clevenger apparatus for 5 hours. Once extraction was completed‚ the resulted essential oil was dehydrated using sodium sulfate (Merck). The essential oil was stored at temperature of 4ºC until using (12). In order to provide different concentrations of the extract‚ Dimethyl sulfoxide (DMSO) was used. Gas chromatography/mass spectrometry analysis of essential oil Isolation and measurement of the sample
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Chm235LL 2/18/14 Caffeine Isolation Lab I. Abstract Solid crude caffeine was extracted from tea and purified via sublimation. The melting point was experimentally found at 215.9-218.7oC. II. Introduction Caffeine is the most dangerous substance being used in lab this semester‚ but it’s also one of the most favorite chemicals commonly ingested among college students. The main purpose of this lab was to extract caffeine from a beaker of about 20 ml of tea. Sublimation was an effective and convenient
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DNA Extraction of a Musa acuminata Biology Lab Report \Analyze and Interpret A number of steps are required to isolate DNA from cellular contents. Describe what happens at each step‚ and why it acts to separate the parts of the cell. /6 There are three specific steps required to isolate DNA from its cellular contents. The steps used to remove and expose DNA from its cell are: breaking down the food type you are using by crushing it‚ for example a banana or strawberries‚ exposing the substance
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of Submission: November 28‚ 2012 Experiment No. 1 ISOLATION OF CAFFEINE FROM TEA I. Introduction Caffeine is a very common substance and is found in coffee‚ tea‚ soft drinks‚ chocolate‚ and "stay-awake" pills such as Vivarin. It can be synthesized or isolated from natural sources. Caffeine constitutes approximately 4% of tea and coffee leaves‚ and is also found in cola nuts and cacao beans. Caffeine is a member of the class of compounds organic chemists call alkaloids. Alkaloids
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Organic Chemistry I – CHEM 201-DL01 September 26‚ 2014 Organic Chemistry Experiment 4B Lab Report Extraction of Organic Compounds From Natural Sources: Extraction of Trimyristin From Nutmeg Introduction The purpose of this experiment is to illustrate how a solid natural product can be extracted from its natural source through the use of an organic solvent. Natural products are organic compounds that are synthesized by natural biological processes in plants‚ animals
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the four different pigments types of a leaf. As the filter paper with the solvent will separate the pigments in terms of solubility‚ a clear segmentation of each is expected to show off. As various chemicals were used in the whole process of this lab‚ certain variables might have influenced the results in terms of the purity of each chemical or purity of the used filtrate. Background The chloroplast‚ basically‚ is the organelle responsible for photosynthesis. Structurally it is very similar to
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