Antibiotics INTRODUCTION Escherichia coli—better known as E. coli—is a gram negative‚ rod shaped bacteria. It is relatively harmless‚ but can occasionally cause food poisoning. It can also provide Vitamin K2. It prevents the establishment of pathogenic bacteria‚ and is associated with or found in the intestinal organ. The antibiotic that E. coli is resistant to is Penicillin. Bacillus subtilis—better known as B. subtilis—is known as the hay bacillus or the grass bacillus. It is gram and catalase
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on the body of marine animals or internal organs of both aquatic and terrestrial animals. Amoeba reproduces asexually by binary and multiple fissions. They eat algae‚ bacteria‚ other protozoan‚ and tiny particles of dead plant or animal matter. Bacillus: They are rod shaped‚ gram positive bacteria found in soil and water. The largest are 2m across and 7m long. Most are aerobic but some under certain conditions can be facultative anaerobes. Each bacterium creates only one spore‚ which is resistant
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(living can only arise from preexisting living) Pasteur ¡V air contained‚ but did not produce‚ microbes (broth‚ s-flasks); pasteurization; lose virility‚ still immunity Lister ¡V aseptic surgery (phenol on surgical wounds) Koch ¡V cause of anthrax (Bacillus anthracis) ¡V bacteria in blood‚ injected into healthy‚ died‚ compared bacteria Jenner ¡V vaccines (cowpox provided immunity against small pox) ¡V milk maids Erlich ¡V ¡§magic bullet¡¨; salvarsan against syphilis Fleming ¡V Penicillium chrysogenum
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Microbiology Exam 1 Name_______________________ 1/30/07 1. (1 pt) Who was the first person to observe bacteria using a microscope? a. Lister b. van Leeuvenhoek c. Pastuer d. Koch 2. (2 pts) Which two of the following contribute to the opportunistic and infectious nature of bacteria? a. flagella b. ability to persist in unfavorable environments c. selectively permeable membranes d. fast growth e. ability to sense chemical gradients f. peptidoglycan 3. (1 pt) Capsules‚ sheaths‚ and
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gram-negative‚ and bacteria that retain the color from the primary stain is called gram-positive. Bacteria stain differently because of chemical and physical differences in the cell wall. Procedure For this lab we used three cultures; Escherichia Coli‚ Bacillus Subtilis‚ and Staphylococcus Epidermidis. Using a different slide for each sample we started by preparing and fixing each slide. Next we covered the slide with crystal violet for 30 seconds then rinsed with water. Next we covered the slide
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Canada Spees Lab 2 Prokaryotes Purpose: Performing this lab will help me better understand the characteristics of prokaryotes and compare them with eukaryotes. This lab will also help me visualize and better understand the physical description‚ and the different types of bacteria and how they stain‚ whether it be gram positive or gram negative. Hypothesis: If I read the textbook and use the resource websites‚ then I will be able to better understand prokaryotes and describe the structural characteristics
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pure bacterial colonies from a mixed population of bacteria. The instructor will provide TSA plates (Tryptic Soy Broth mixed with 15% agar‚ heated to sterilize media and melt agar then poured into a Petri dish) and a culture containing Escherichia coli and/or Bacillus subtilis and/or Staphylococcus aureus and/or Serratia marcescens. Isolation of bacteria using the streak plate technique: Label the bottom of a Petri dish (side containing the agar) (Why?) with your initials‚ date and lab section
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CHAPTER I INTRODUCTION 1.1 Background Food is one of human sources of calorie‚ protein‚ fats‚ and nutrition. Yet‚ because of the highly nutritious content‚ food is susceptible to growth of microorganisms. By the presence of microorganisms in food‚ the food is more likely to have shorter shelf life. Thus‚ mostly it is resolved by the addition of antimicrobial substances to food‚ such as condiments and preservatives. Condiments and preservatives could inhibit the growth of microorganisms
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activity of Sudanese Cucumis melo L. fixed oil was investigated. Agar cup diffusion assay was performed against two fungal species (Candida albicans and Aspergillus niger)‚ two gram positive bacteria (Staphylococcus aureus and Bacillus subtilis) and two gram negative bacteria (Escherichia coli and Pseudomonas aeruginosa). Ampicillin‚ gentamycin and clotrimazole were used as positive controls. Antimicrobial activity was shown by all the species except E.coli and P. aeruginosa. Oil showed a highest activity
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Objectives 1. To determine biochemical activities of microorganisms. 2. To observe the product of biochemical activities of microorganisms. 3. To learn the skills of inoculation agar tubes and agar plates. Introduction Microorganisms are able to carry out different biochemical activities with the ease of different enzymes. Each of these enzymes carries out one specific type of the chemical transformation. They convert substrates into product. A) Carbohydrates Fermentation Microorganisms utilize
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