GRAM STAINING EXPERIMENT CONDUCTED ON 9/29/2013 Introduction: The Gram stain is a useful stain for identifying and classifying bacteria. The Gram stain is a differential stain that allows you to classify bacteria as either gram positive or gram negative. This gram stain technique was discovered by Hans Christina Gram in 1884. The gram stain procedure separates all bacteria into one of two groups - into gram-negative bacteria which do not stain purple and into gram-positive
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Microbiology: ‘The Correct handling of Micro-organisms’ 1. Devise a title for each of the two experiments you did : (i)‚ Experiment 1 demonstrated the growth of bacteria when placed in liquid nutrient broth culture‚ the number of species present had increased in growth. .(1) (ii) Experiment 2 illustrated the growth of bacteria when placed on different surfaces of solid agar plates which included: nutrient agar‚ CLED agar and MacConkey agar; the number of species present also had increased in growth
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younger than 2 years. Newborns with pneumonia commonly present with poor feeding and irritability‚ as well as tachypnea‚ retractions‚ grunting‚ and hypoxemia. Infections with group B Streptococcus‚ Listeria monocytogenes‚ or gram-negative rods (eg‚ Escherichia coli‚ Klebsiella pneumoniae) are common causes of bacterial pneumonia. Group B streptococci infections are most often transmitted to the fetus in utero. The most commonly isolated virus is respiratory syncytial virus (RSV). Cough is the most common
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had the following morphology on a streak plate: medium sized butyrous cream colored colony. Gram-staining was utilized as a first step to differentiate between gram-positive and gram-negative. After determining that it was a Gram negative single bacillus‚ biochemical tests performed and their purpose and results are shown in table and flow chart form. Table 1: biochemical Test Results Test | Purpose | Reagents | Observations | Results | Gram stain | To determine the Gram reaction of the bacterium
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negative. Through this experiment‚ gram staining skills develop. More understanding the types and morphology of bacteria. Expected experimental result‚ Escherichia coli (E.coli ) is a negative gram bacteria which stain pink colour ‚ while Staphylococcus aureus ( S.aureus ) is a positive gram bacteria which stain purple colour. Materials Bacteria : Escherichia coli ‚ Staphylococcus aureus Crystal Violet Gram’s iodine Absolute alcohol Safranin Methodology As per manual. Discussion Gram staining
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However‚ a few species — for example‚ Thiomargarita namibiensis and Epulopiscium fishelsoni — are up to half a millimetre long and are visible to the unaided eye; E. fishelsoni reaches 0.7 mm. Among the smallest bacteria are members of the genus Mycoplasma‚ which measure only 0.3 micrometres‚ as small as the largest viruses. Some bacteria may be even smaller‚
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Introduction to Microbiology Laboratory report №10 Physical factors affecting growth microbes: Temperature‚ pH and oxygen requirement. Student: Temirlan Aitbekov Lab partner: Kanat Sadykov Instructor: Alessandra Clementi‚ MD‚GP Lab date: 7/11/14 Due date: 14/11/14 Nazarbayev University Abstract: This experiment is directed
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1. BIOLOGICAL CLASSIFICATION Aristotle’s classification - Aristotle was the earliest to attempt a more scientific basis for classification of organisms. - He classified plants into trees‚ shrubs & herbs and animals into two groups‚ those which had red blood and those that did not. Two-kingdom classification - In Linnaeus’ time Two Kingdom classification (Kingdom Plantae & Kingdom Animalia) was developed. Drawbacks of 2-kingdom classification Prokaryotes (Bacteria‚ cyanobacteria) and eukaryotes
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measuring zone of inhibition. Inhibiting concentrations used for all the extracts was 500 mg/ml. Methanolic extract of C. album was found to be most active among all other extracts and inhibits Staphylococcus epidermidis‚ Bacillus subtilis‚ Staphylococcus aureus‚ Escherichia coli. The extract of a mixture of solvents was also found to be effective in inhibiting Staphylococcus aureus. Methanolic extract of C. album was found to give a most clear zone of inhibition. The antibacterial activity is attributed
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Chemotherapeutic Agents of Control: Introduction: Chemotherapeutic agents are chemical substances used to treat various forms of infectious diseases. The chemotherapeutic agent works by stopping the bacteria from reproducing. There are two different kinds of chemotherapeutic agents. The first kind is an antibiotic‚ which slow down the growth of microorganisms. The second kind of chemotherapeutic agent is synthetic drugs. Synthetic drugs are artificially made in a laboratory. Chemotherapy
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