Bacillus Cereus Bacillus cereus is a rod-shaped gram- positive bacillus that can be found in food‚ dust‚ dirt and sometimes soil. It is an aerobe and a facultative anaerobe that can form spores‚ which if activated at any point can begin to germinate and produce toxins. These spores that form from B. cereus are be resistant to heat and chemicals. B. cereus is found in approximately 25% of food sampled (3). Foods that are commonly associated with B. cereus are potatoes‚ rice‚ meat‚ vegetables
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I had # 8 organism‚ Bacillus subtilis. Bacillus subtilis is a Gram-positive bacteria‚ rod-shaped and catalase positive. In order to figure out what is my unknown organism for this assignment‚ I had to perform series of tests. First was the glucose‚ lactose‚ and sucrose test. Through these three tests‚ I was able to detect the ability of the microorganism to ferment a specific carbohydrate. Fermentation reactions are observed by the color change in pH. In class‚ we used phenol red as the pH indicator
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Bacillus thuringiensis Bacillus thuringiensis Spores and bipyramidal crystals of Bacillus thuringiensis morrisoni strain T08025 Scientific classification Kingdom: Eubacteria Phylum: Firmicutes Class: Bacilli Order: Bacillales Family: Bacillaceae Genus: Bacillus Species: thuringiensis Binomial name Bacillus thuringiensis Berliner 1915 Contents Introduction Use in pest control Genetic engineering for pest control Usage Advantages Safety Limitations to Bt crops
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INTRODUCTION Bacillus anthracis has recently become popular as the causative agent of anthrax infections. Belonging to the bacillus species‚ the bacterium is considered‚ along with Bacillus cereus‚ to be of extreme medical importance. Until recently‚ the condition caused by the bacterium was rarely heard of. Although the bacterium is naturally found in the soil‚ its rare occurrences in humans have only been observed in those working with livestock or imported livestock products‚ therefore the
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Heat resistance of endospores of Bacillus pumilus Abstract Spore suspension of Bacillus pumilus was inoculated into universal bottles containing sterile distilled water in water baths at temperatures of 85°C‚ 90°C and 95°C. At specific time intervals‚ a sample was removed and spread on nutrient agar plates. The number of colonies formed was used to determine the D-value and z-value. The D-value for 85°C is 64.1 minutes‚ 25.7 minutes for 90°C and 8.2 minutes for 95°C while the z-value is 11
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animals being utilized in animal by-products and thereby to reveal when these products contained ingredients originating from animals that had died of anthrax. The thermostable antigens involved are common to other Bacillus species so the test depends on the fact that the only Bacillus likely to have proliferated within and throughout an animal depositing extensive precipitating antigens in the tissues is B. anthracis. Procedure: 1. Chop or slice the specimen into fine pieces or strips. 2
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Microbiology Coursework: Bacillus cereus After investigation following on outbreak of food poisoning at a pizza restaurant‚ it was found that all suffers had consumed a portion of side salad from the self-service salad bar alongside their main dish. Subsequently‚ this was further traced to a rice salad. Environmental Health Officers investigating this outbreak suspected it may have been caused by Bacillus cereus (B. cereus). The presence of large numbers of B. cereus in a food is indicative
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The Identification of Bacillus Badius [pic] Hannah Melanchuk Dr. Robert Suran May 1 2013 Microbiology Abstract Over a three week time span I was able to successfully identify an unknown microorganism by combining the results of several tests. The first test was the Gram stain test which showed the microorganism was Gram positive and rod shaped. Using an Unknown Identification Flowchart‚ I proceeded to inoculate a Starch plate. In week 2‚ I
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biochemical reactions. Qualitative observations were made on the tests in identification process. The data from experimentation provided sufficient results to suggest that the identity of unknown bacterium was the gram-positive spore forming bacteria‚ Bacillus Subtilis. Introduction. One of the main responsibilities of a medical lab is to determine the identity of pathogenic bacteria. It is important to determine the specific type of bacterium causing disease so the physician is able to correctly
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culture from the agar cultures 3. It then mix with the drop of water and then proceeds as broth cultures. SIMPLE STAINING TECHNIQUES Material: 1. 24 hours broth cultures of a. E. coli b. Staph. aureus 2. 24 hours nutrient agar slants of a. Bacillus subtilis b. Pseudomonas aeruginosa 3. Slides 4. Inoculating loop 5. Dye solution a. Crystal violet b. Methylene blue c. Carbol fuchsin 6. Test tubes Procedure: 1. By using inoculating loop a minute of bacterial was transferred onto the
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