Differential Staining Cynthia Alonzo‚ M.S. Version 42-0242-00-01 Lab RepoRt assistant This document is not meant to be a substitute for a formal laboratory report. The Lab Report Assistant is simply a summary of the experiment’s questions‚ diagrams if needed‚ and data tables that should be addressed in a formal lab report. The intent is to facilitate students’ writing of lab reports by providing this information
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1. Nutrient agar plates of a) Sarcina lutea and Serratia marcescens b) Bacillus subtilis and Escherichia coli c) Streptococcus fecalis and Pseudomonas fluoroscens 2. 24 hour pour plate preparation of a) Serratia marcescens b) Sarcina lutea c) Bacillus subtilis d) Escherichia coli 3. 24 hour agar slant of the following cultures incubated at 25ºC a) Bacillus subtilis b) Escherichia coli c) Serratia marcescens d) Pseudomonas fluoroscens 4. 48 hour nutrient
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the Fermentation of Lactose Test‚ the Sulfide Indole Mobility (SIM) Test‚ the Nitrate Reduction Test‚ the Protein Hydrolysis Test‚ the Catalase Test‚ and the Cytochrome Oxidase Test. The microbes that were tested during this lab were: Escherichia coli‚ Bacillus cereus‚ the unknown‚ Proteus vulgaris‚ Staphylococcus epidermis‚ Enterobacter aerogenes‚ the control‚ and Pseudomonas fluorescens. The microbes tested during these various tests were looking for which would: reduce sulfur/produce sulfate
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purpose of the two experiments was to determine the fundamental effects that temperature has on the growth and survival of bacteria. During the first experiment five different bacterial broth cultures of Escherichia coli‚ Pseudomonas fluorescens‚ Enterococcus faecalis‚ Bacillus subtilis and Bacillus stearothermophilus were individually incubated at temperatures of 5‚ 25‚ 37‚ 45 and 55°C for one week in an aim to distinguish the effect temperature has on growth and survival of the five different species
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DEVELOPING A CONVENIENT ANALYTICAL TECHNIQUE FOR THE QUANTITATIVE ESTIMATION OF RESIDUAL CTAB PRESENT IN HEAMOPHILUS INFUENZAE type b POLYSACCHARIDE Thesis submitted for the degree of Bachelor of Technology Biotechnology 1 BY 2 Mr. Sarthak Ranka 2 Under the guidance of 1 Dr. SURESH BERI ADDITIONAL DIRECTOR (PRODUCTION) Serum Institute Of India Ltd 212/2‚ Hadapsar Pune (MH)-411028 1 Submitted to
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Hydrolysis Report Bile Esculin Hydrolysis‚ Starch Hydrolysis‚ UreaHydrolysis‚ Casein Hydrolysis Hydrolysis Report Introduction Hydrolysis is a chemical reaction that uses water to split complex molecules. The water molecule H2O is split in the mechanism of hydrolysis‚ hydrogen cations and hydroxide anions. When the enzyme catalyzes its reaction inside the cell‚ it is referred to as intracellular hydrolases. When the enzymes secreted from the organism to catalyze reactions outside the cell‚ it
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Introduction In this experiment‚ the growth of four cultures‚ which are Escherichia coli‚ Staphylococcus aureus‚ Saccharomyces cerevisiae (yeast) and Penicillum (mold) were examined under a range of acidic to alkaline environments to determine the pH requirements of the four species. By conducting this methodology‚ the range of acceptable pH limits‚ including the optimum pH for each organism‚ can be determined. The range of pH over which an organism grows is defined by three cardinal points: the minimum
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Lab Report: Antibiotic Evaluation by the Kirby-Bauer Method Introduction Chemical antimicrobial agents are chemical compounds capable of either inhibiting the growth of microorganisms or killing them outright. Those which are taken internally to alleviate the symptoms of or promote healing from disease are called chemotherapeutic drugs‚ and among these is a class of compounds called antibiotics. In order for a chemotherapeutic drug to be classed as an antibiotic‚ it must be produced by
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sterile loop‚ we streaked a single line of each culture Bacillus subtilis‚ Pseudomonal aeruginosa‚ and Escherichia coli‚ to each of the separate sections. The plate was incubated until the next lab period‚ where we then flooded the plate with Iodine and were able to observe and record starch Hydrolysis. (Table 1) * In order to test carbohydrate catabolism under anaerobic conditions we tested cultures of Pseudomonas aerugenosa‚ Escherichia coli‚ and Alcaligenes faecalis in tubes containing a glucose
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poisoning‚ while Streptococcus pyogenes may cause tonsillitis and strep throat. Bacillus Bacillus refers to rod shaped bacteria that are usually Gram-positive or negative. E. coli and Salmonella are the most commonly encountered species responsible for food poisoning and typhoid fever‚ respectively. According to the University at Albany‚ bacillus also includes two of the most dangerous bacteria known‚ that is Bacillus anthracis‚ which causes the lung disease anthrax‚ and Clostridium‚ which causes
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