Microbiology Coursework: Bacillus cereus After investigation following on outbreak of food poisoning at a pizza restaurant‚ it was found that all suffers had consumed a portion of side salad from the self-service salad bar alongside their main dish. Subsequently‚ this was further traced to a rice salad. Environmental Health Officers investigating this outbreak suspected it may have been caused by Bacillus cereus (B. cereus). The presence of large numbers of B. cereus in a food is indicative
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I had # 8 organism‚ Bacillus subtilis. Bacillus subtilis is a Gram-positive bacteria‚ rod-shaped and catalase positive. In order to figure out what is my unknown organism for this assignment‚ I had to perform series of tests. First was the glucose‚ lactose‚ and sucrose test. Through these three tests‚ I was able to detect the ability of the microorganism to ferment a specific carbohydrate. Fermentation reactions are observed by the color change in pH. In class‚ we used phenol red as the pH indicator
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The Life of Escherichia Coli Escherichia Coli 0157:H7 bacterium can live in the intestines of cattle‚ therefore meat can become infected during slaughtering. You can contract this bacterium by eating undercooked meat‚ especially ground beef. Escherichia Coli 0157:H7 can also be contracted by eating contaminated fruits or vegetables‚ as well by drinking raw fruit juices or milk‚ or sewage contaminated water. Infection can also be spread via person to person. Escherichia Coli 0157:H7 can also
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Identifying the Unknown Bacteria: Bacillus cereus Brittany Moy Coastline Community College Abstract Bacillus cereus is a Gram-positive‚ facultative aerobic‚ spore-forming‚ rod-shaped bacterium that is widely distributed among the environment. Introduction The reason for identification of unknown bacteria was to help students recognize different bacteria through different biochemical tests and characteristics. This is important in the medical field because identification of unknown bacteria
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Systematic Identification of Bacillus subtilis and Serratia marcescens Through a Battery of Tests and Plates Introduction: The purpose of this experiment was to use a systematic battery of tube tests and plates designed to lead to identification of two unknown bacterial species‚ from the combination of all results. A sample of bacteria was used‚ labeled “Sample 4”‚ from which both species was to be obtained‚ one gram positive and one gram negative. Table 1 is a list of the possible bacteria to
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Escherichia coli and Staphylococcus albus Some bacteria’s require a certain amount of light in order to function properly and there fore survive. Escherichia coli is found in the gut and intestines of mammals. Staphylococcus albus is generally a skin dwelling bacteria Aim: To test whether the amount and type of light that the bacteria’s E.coli and S.albus are subject to causes changes in their growth rates and habits. To test whether the growth rates and habits of the bacteria’s E. coli
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but then over time it should increase more rapidly throughout what is called the log phase. This predictable pattern of growth can be called the organism’s biotic potation. Procedure During this exercise the population growth of the bacteria Escherichia coli (E.coli) will be measured. If the conditions are right‚ the E.coli population will grow more rapidly the longer it stays in that area. Turbidity‚ meaning cloudiness‚ will be how the tubes are measured to give the results of bacterial growth
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Transformation Of Escherichia Coli With pGLO Plasmid April 24‚ 2013 ABSTRACT: This experiment focuses on genetic engineering and transformation of bacteria. The characteristics of bacteria are altered from an external source to allow them to express a new trait‚ in this case antibiotic resistance. In is experiment foreign DNA is inserted into Escherichia coli in order to alter its phenotype. The goal of the experiment is to transform E. coli with pGLO plasmid‚ which carries a gene for ampicillin
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Abstract The purpose of this experiment was to study the transfer of genetic information on plasmid F’lac by using Escherichia coli. Plasmid transfer was measured by using two different methods. The first one was by using selection and contraselection with three antibiotics: streptomycin(which was replaced by naladixic acid for the second part of the experiment)‚ampicillin and kanamycin and the second one by using a colour indicator ( X-gal). As significant results‚ the percentage of transfer for
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bacterium Escherichia coli is being subjected to various environmental factors that affect the rate of growth. These factors scrutinized were the different types of nutrients‚ the intensity of aeration‚ or the temperature at which it was stored. The purpose of this lab is to determine which factor affects the Escherichia coli the greatest. It is known that these abiotic factors affect the rate of growth the greatest if they remain at the correct conditions for living. Escherichia coli and other
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