(TSI) slant is used to identify sugar fermentation in a microorganism; it contains a red pH-sensitive dye that will turn yellow under acidic conditions‚ such as contact with the acidic byproducts of sugar fermentation. The three sugars‚ sucrose‚ lactose and glucose‚ are present in specific concentrations‚ 1%‚ 1%‚ and .1% respectively (Hajna‚ 1945). The combination of the color change results and the location in the tube of the changes allows for a multitude of varying results. The TSI slant is a
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dehydrogenase is very stable and is found at high concentration only in the mitochondrion of eukaryotes. It can be easily used as a marker enzyme for mitochondria and we used it to estimate the realative amounts of mitochondira in…Jacob and Monod used the lactose metabolism system of Escherichia Coli (E. Coli) to better understand the problem of enzyme induction‚ the occurrence of a particular enzyme only when its specific substrate is present. In the lac operon system‚ enzyme induction occurs when the presence
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had # 8 organism‚ Bacillus subtilis. Bacillus subtilis is a Gram-positive bacteria‚ rod-shaped and catalase positive. In order to figure out what is my unknown organism for this assignment‚ I had to perform series of tests. First was the glucose‚ lactose‚ and sucrose test. Through these three tests‚ I was able to detect the ability of the microorganism to ferment a specific carbohydrate. Fermentation reactions are observed by the color change in pH. In class‚ we used phenol red as the pH indicator
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on U45 based on instruction given in Microbiology Lab Manual by John P. Harley‚ and by the instructor: 1. Carbohydrate fermentation 2. Sulfide Indole Motility (SIM) test 3.
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appearance of bright pink is a positive test for a urease-producing bacteria. Materials include a urea-containing medium‚ and a pure culture of bacteria. The litmus milk reactions are used to determin whether bacteria have the ability to utilize lactose‚ protein‚ and litmus or not. The litmus serves as a pH indicator which turns pink in the presence of acid and blue in alkaline. If sufficient acid is produced‚ casein will be denatured and form a firm curd and whey which is a clear‚ watery fluid at
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was used in a way to help recognize those specifics and identify the unknown cultures. The differential tests used to identify the unknown cultures were oxidase‚ catalase‚ lactose and sucrose fermentation‚ Kugler/iron agar‚ nitrate reduction‚ gelatin hydrolysis‚ starch hydrolysis‚ manitol salt‚ MR-VP‚ citrate‚ bile esculin‚ indole‚ urease‚ DNase‚ and coagulase. Material & Methods The tests performed on the unknown bacteria cultures were all used to determine the identity of the bacteria.
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Microbiology Laboratory Report Identification of Unknown Bacteria 03/10/05- 04/01/05 Authors: Richard Hendricks‚ Jessica Prebish; NMU Abstract: Broth culture 16 was randomly selected by our group and subjected to qualitative tests for taxonomic identification. The culture did appear homogenous throughout the testing period and is currently retained by Northern Michigan University’s department of Microbiology. We suggest that culture 16 is an example of Escherichia coli. Background: Techniques
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Proteus vulgaris #12 The importance of identification of a certain microorganisms can range between a life threatening diseases to a creation of certain antibiotic. Understanding the principals of living microbes and identifying my unknown bacteria through numerous biochemical and metabolism tests‚ with the outmost confidence‚ Proteus vulgaris had the precise qualifications. The point of this report is to further explore the identification of my unknown bacteria by revealing the results of the
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to distinguish six different microbes from one another and to compare how their metabolic and biochemical processes differ from species to species to determine the unknown sample. The tests included: Triple sugar iron agar (TSAI)‚ the Sulfide Indole Mobility (SIM) test‚ Glucose fermentation‚ the Methyl Red test‚ the Voges-Proskauer test‚ Citrate test‚ the Urease Test‚ and finally the Gelatin test. The microbes that were tested during this lab were: Escherichia coli‚ Enterobacter aerogenes‚
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environment meaning more animals can stop their and release their feces‚ and also the animals that reside there continue to reproduce‚ leaving no exit for their excretions. By using the MPN test‚ I was able to detect the coliforms from the fermentation in lactose broths‚ then with the IMViC test I was able to identify the enteric bacteria colonizing in the water samples. By then end of the experiment‚ it was apparent that the USM Creek and Leaf River were the two that tested positive for possible
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