Columbia colistin nalidixic acid agar (Columbia CNA) with 5% sheep blood as my medium. I used the same streak plate technique on this medium as I used on the TSA plates and incubated it at 37°C as well. Then‚ I used MacConkey agar as a medium to detect lactose fermentation‚ used the same streak plate technique with my bacteria‚ and incubated the plate at
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Influence of Malonate and Sodium Fluoride on the Activity of Enzyme Succinate Dehydrogenase to Assess Inhibitors of Cellular Respiration Thaovy Mai Tran BI 151.5233 (Winter 2013) Todd Tiano 03/25/2013 Introduction Most organisms produce adenosine triphosphate (ATP) as a source of energy for cellular work‚ using cellular respiration. Cellular respiration
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Isolation and identification of pathogenic bacteria associated with frozen mackerel fish (Scomber scombrus) in a humid tropical environment Eze‚ E. I. 1 ‚ Echezona‚ B. C. 1 * and Uzodinma‚ E. C. 2 1 Department of Crop Science‚ University of Nigeria‚ Nsukka‚ Nigeria. 2 Department of Science Laboratory Technology‚ University of Nigeria‚ Nsukka‚ Nigeria. Accepted 8 January‚ 2010 Aquaculture products can harbour pathogenic bacteria which are part of the natural micro-flora of the environment. An in-vitro
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Lab12-Medical Microbiology- Part1– Differential Media/Biochemical Tests‚ Sp2012 (Set all of your margins to ½”) Purpose: The purpose of this lab is to help you become a little familiar with some of the tests that can be typically performed in a clinical or research lab facility. These tests may help in determining a particular pathogen’s growth needs. There are several sections to this lab. Find each section and complete the “Preparing for Class” sections. Preparing for class - Day 1 Read
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Introduction The purpose to this lab was to isolate and identify two unknown bacteria from a mixed culture provided to us by our instructor. This study was done by applying all of the methods that have been instructed on thus far in microbiology laboratory class. Each test performed‚ provided us with some key information about the unknown microbes in question and how the bacteria function. Materials and Methods Over a two week period‚ eight prepared types of test media were provided to
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We chose to test the mitochondria under the presence of malonate because from research we discovered that malonate is the specific inhibitor for succinate dehydrogenase (C. A. Baumann and F. J. Stare). The inhibitor in this case will slow down or stop the catalysis action of the SDH enzyme causing the cuvette tubes to become more colorless
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ABSTRACT In todays world of ever evolving genetic mutations the incidence of drug resistant forms of microorganisms are on the rise. The importance of identifying these pathogens and their related epidemiology has become increasingly more important. The purpose of this study was to identify an unknown bacterium in a controlled laboratory environment over a 5 week period. Utilizing a variety of differential testing and staining methods learned in the microbiology course‚ students were to determine
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Any sign of yellow should be inter preted as a positive reaction‚ orange should be considered negative. Lactose Bacterial fermentation of lactose‚ which results in the formation of acidic end products‚ is indicated by a change in color of the Indicator present in the medium from red (alkaline) to yellow (acidic). Any sign of yellow should be interpreted as a positive reaction;
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Introduction: Being able to identify a particular bacterial species is important. It is very useful in knowing its risk of toxicity to humans or animals‚ its resistance or susceptibility to antibiotics‚ and determining how to control its growth or kill it altogether. The purpose of these procedures is to discovery the identity of an unknown microbe by observing its reactions to a barrage of chemical and physical tests. Different microorganisms react in different ways‚ due to their function‚ digestibility
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Positive control – M. smeagmatis Blue – negative Pink /red- positive 4. Motility Positive control - P.vulgaris A. Non motile is negative test B. Motile is a positive test 5. Carbohydrates fermentation (test for gram -) ( glucose ‚ manitol‚ lactose) Positive control – E.coli (yellow) Red- no color change – negative test Yellow – color change – positive test 6. MaConkey’s Agar Positive control - E.coli Selective for negative gram stain . Differential for organism that could ferment lactase
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