Purpose: To identify an unknown bacterial specimen using basic laboratory technique and biochemical tests. The unknown bacteria will be one of the following: Enterococcus faecalis‚ Staphylococcus saprophyticus‚ Escherichia coli‚ Enterobacter aerogenes‚ Proteus vulgaris‚ Salmonella [I assume typhimurium]‚ or Shigella [either flexneri or sonnei‚ we used both in our lab during the semester]. Procedure {and observations}: Observe bacterial colony morphology. {Colonies are large‚ beige or
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6-dichloroindophenol (DCIP)‚ and 10 mM succinate in 50 mM NaPO4 pH 7.4 The final concentrations of malonate used were: 1mM‚ 2mM‚ and 3mM and were used in tubes 3-6. SDH activity was measured against malonate activity‚ since malonate was expected to inhibit SDH activity. The control used in this assay was used to determine the background amount of SDH activity‚ using the same setup bit no succinate in 50 mM NaPO4 pH 7.4 or malonate was added to the control. After the samples were prepared‚ 250 μl of homogenate was
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* Swabbing stick * Principle Procedure TSI (TRIPLE SUGAR IRON TEST) Materials * TSI agar * Straight wire loop * Cultured colony of organism * Purity plate Principle TSI agar detects fermentation of glucose 0.1%‚ lactose 1% and sucrose 1%‚ production of H2S‚ and gas formation. The medium contains ferric ammonium citrate‚ and phenol red indicator Procedure * Take half of an isolated colony and stab the butt of the agar and make streaks on the slant. * Close
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The tubes were incubated at 35°C for 24 hours‚ following which‚ the cloudiness and bubble trapped in Durham tube was continued for further analysis. Next‚ 1 ml of each tube of LST were transferred into tube of 9 ml of Brillian Green Lactose Bile (BGLB) for confirmation of total coliform. BGLB tubes were incubated at 35°C and gas production was examined after 48 hour. The confirmations were determined by the cloudiness and trapped bubble in the Durham tube (FDA‚
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to the family Enterobacteriaceae. The historical definition of this group has been based on the method used for detection (lactose fermentation) rather than on the tenets of systematic bacteriology. Accordingly‚ when the fermentation technique is used‚ this group is defined as all facultative anaerobic‚ gram-negative‚ non-spore-forming‚ rod-shaped bacteria that ferment lactose with gas and acid formation within 48 h at 35°C. The standard test for the coliform group may be carried out either by the
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ANTIMICROBIAL ACTIVITY OF SOIL USED IN THE TREATMENT OF BACTERIAL DIARRHOEABYSITUMBEKO LIWELEYA(s213459531)Research Proposal Submitted in fulfilment of the requirements for the degree of BTECHBIOMEDICAL TECHNOLOGY | | FACULTY OF HEALTH SCIENCES | atNelson Mandela Metropolitan University | MODULE- RESEARCH METHODOLOGYDUE DATE- 16th MAY‚ 2013.SUPERVISOR- PROFESSOR N. SMITH | | Contents ANTIMICROBIAL PROPERTIES OF SOIL USED IN THE TREATMENT OF BACTERIAL DIARRHOEA
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Isolation and identification of an unknown bacterium Pillay‚ Esmerelda (209504371) School of Biochemistry‚ Genetics and Microbiology Department of Microbiology University of Kwa-zulu Natal 25 October 2010 ABSTRACT Different types of bacteria in various forms are found all around us‚ and it is a microbiologist’s job to be able to identify these bacteria. Using various staining techniques and physiological tests‚ an isolated bacterium can be identified. In this experiment‚ a single bacterial
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Having the ability to collect and identify unknown microorganisms is vital in health and medicine. This capability is important for a variety of reasons‚ such as knowing the causative agent of disease‚ knowing if the microorganism obtains any beneficial properties and knowing the correct microorganism to use to create a successful antibiotic. Implementing the experimental methods learned thus far in the microbiology laboratory allowed an unknown bacterium to be identified as a result of this study
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and a few other carbohydrates‚ with the production of acid and usually gas. As for the unknown‚ it was able to ferment glucose (without the production of gas‚ as the Durham tube placed inside the PR Glucose was not displaced) and sucrose‚ but not lactose. The abovementioned genus is also indicated to produce a negative result in oxidase test and a positive result in catalase test‚ and the unknown produced negative and positive results in those tests‚ respectively. The genus is also indicated in the
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CHEM 2018 Organic Lab Part 2 Experiment 7: Diethyl n- Butylmalonate Lab Partner: Reference: Williamson K.L.‚ & Masters
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