Weekly iLab iLab: Bacterial Growth and Controlling Bacterial Growth Scenario/Summary: This week’s experiment focuses on bacterial growth and methods to control bacterial growth. This week’s experiment will be conducted in two parts: Experiment I: Growth Media and Patterns of Growth and Experiment II: Controlling Microbial Growth. Experiment I: Growth Media and Patterns of Growth. In part I of this week’s experiment‚ you will explore the characteristics of several types of growth media. For example
Premium Bacteria Microbiology Microorganism
Gram-negative bacterium‚ the unknown bacterium’s ability to ferment lactose was analyzed first. Positive lactose fermentation was indicated on both the MacConkey agar plate and SSA by the growth of bright pink colored colonies (Table 2; Kenyon College‚ 2011; Lancaster and Bennett‚ 2012). Identical results were also found in the inoculated lactose broth with the Durham tube and the TSI tests‚ which both indicated positive fermentation for lactose as well (Lancaster and Bennett‚ 2012; Watson‚ 2013;
Premium Bacteria Microbiology Escherichia coli
Salmonella enterica (#11) Gram Negative Organism | CarbohydrateFermentation(PRL) | CarbohydrateFermentation(PRG) | Indole productionTest | MethylRed Test(MR) | VogesProskauer Test | Citrate Utilization Test | Hydrogen sulfide Test | Urease Test | CatalaseTest | Salmonellaenterica | (-)Red color | (+)YellowColor | (-)No red ring | (+)RedRing | (-)No pink | (+)Blue With growth | (+)Black PrecipitateVery motile | (-)No change in color | (+) | The unknown organism (#11) was Salmonella
Premium Gram staining Bacteria Enzyme
incapable of fermenting glucose cannot utilize lactose. 2 enzymes necessary for a bacterium to take up lactose: A. β-galactoside permease – facilitates the entry of lactose molecules to bac cell wall B. β-galactosidase – breaks down lactose into β-D glucose and β-D galacatose LF possess both enzymes NLF do not possess β-galactosidase LLF do not possess β-galactoside permease Glucose fermenters only (true enteric pathogen) Glucose and lactose fermenter (most opportunistic enterics)
Premium Hydrogen sulfide PH indicator Bacteria
Determining an Unknown Through Deferential Stains and Biochemical Tests Introduction There are many reasons for knowing the identity of microorganisms. The reasons range from knowing the causative agent of a disease in a patient‚ so as to know how it can be treated‚ to knowing the correct microorganism to be used for making certain foods or antibiotics. This study was done by applying all of the methods that have been learned so far in the microbiology laboratory class for the identification
Premium
the bottom of the tube‚ and litmus has formed a pink band at the surface of the clear liquid as well. * -Coagulation 65. Describe the physical change that occurs during lactose fermentation. Name one microbe capable of producing this reaction. * Lactose Fermentation involves the initial breakdown of lactose to glucose and the subsequent fermentation of glucose to produce an acid end product. 66. Describe the physical change that occurs during an alkaline reaction. Name one microbe
Premium Hydrogen Metabolism Bacteria
double-strength and 10 single-strength lactose broths with our group information‚ section number and which sample we were testing. We labeled five of the single-strength tubes 1.0ml and the other five 0.1ml. We then shook up the water sample vigorously and then transferred 10ml of the water to each of the five double-strength lactose broths‚ 1.0ml of the water to the single-strength lactose labeled 1.0ml‚ and 0.1ml of the water to the single-strength lactose labeled 0.1ml. After transferring the
Premium Bacteria Microbiology Escherichia coli
in order to determine the identity of the unknown organism. The tests performed to identify the unknown organism included Gram Staining‚ Fluid thioglycollate/aero-tolerance test‚ Methyl Red-Vogues Proskauer‚ SIM test‚ Lysine Decarboxylase test‚ Lactose Fermentation test‚ Simmons Citrate test‚ and TSI test. The Gram staining technique showed that the organism was Gram negative and bacilli shaped. The fluid thioglycollate test showed that the organism was a facultative anaerobe‚ meaning ATP can
Premium Microbiology Bacteria Gram staining
to identify their bacteria. During the process of identification‚ the unknown bacteria was added to many different testing medias using aseptic technique. They are as follows: lactose fermentation on eosin methylene blue (EMB)‚ TSI (Triple Sugar Iron agar)‚ Phenol red sucrose‚ the SIM test‚ H2S by SIM‚ IMViC (indole‚ motility‚ voges-proskauer‚ and citrate)‚ Urease (urea broth)‚ PDase (Phenylalanine Deaminase)‚ Lysine Decarboxylase‚ and Ornithine Decarboxylase. Colonial morphology on EMB was used
Premium Escherichia coli
UNKNOWN LAB REPORT UNKNOWN NUMBER 54 Ron Williams 7-28-05 Bio 205 Prof. Curlee Fall 2005 Purpose There are many reasons for knowing the identity of microorganisms. The reasons range from the knowing the causative agent of a disease in a patient‚ so as to know how it can be treated‚ to knowing the correct microorganism to be used for making certain foods or antibiotics. This study was done by applying all of the methods that have been learned so far in the microbiology laboratory
Premium Bacteria Enzyme Glucose