differentiate microbes from one another and to compare how metabolic and biochemical processes differ from species to species. The tests performed include: the Fermentation of Sugars Test (sucrose‚ glucose‚ and lactose)‚ the Urease Test‚ the Fermentation of Lactose Test‚ the Sulfide Indole Mobility (SIM) Test‚ the Nitrate Reduction Test‚ the Protein Hydrolysis Test‚ the Catalase Test‚ and the Cytochrome Oxidase Test. The microbes that were tested during this lab were: Escherichia coli‚ Bacillus
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applied. The first procedure that was done was the gram stain. Under a microscope‚ if the gram stain is purple‚ the bacterium is gram positive‚ if the stain is red‚ it is gram negative. The next test was the fermentation tests for glucose‚ sucrose and lactose. If an organism is able to ferment the carbohydrate‚ there will be acid produced‚ which will turn the broth yellow. Homofermentation shows that only one product was produced-acid. Other organisms will carry out heterofermentation‚ the presence of
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according to the index of the Virtual Unknown software and the following tests were performed: 1) Adonitol Fermentation 2) Cellobiose Fermentation 3) Maltose Fermentation 4) Lactose Fermentation 5) Raffinose Fermentation 6) Sorbitol Fermentation 7) Sucrose Fermentation 8) Melibiose Fermentation 9) Indole Production Results Table 1: Biochemical Tests Results Test | Purpose | Reagents | Observations | Results | Adonitol Fermentation | To determine if the microbe can ferment
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1. Title: Double unknown identification of a mixed culture of Gram-negative and Gram-positive bacteria 2. Author: Nick Fiore‚ University of Kansas‚ Biology 402‚ Fall 2014 3:00pm room 6040 3. Abstract: The purpose of this experiment was to isolate two unknown bacteria and perform a series of selective and differential tests to correctly identify each. After the bacteria was isolated a series of differential and selective tests following the dichotomous key attached were used to identify each bacteria
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HOW TO WRITE AN UNKNOWN LAB REPORT IN MICROBIOLOGY GENERAL Unknown reports in microbiology are written in scientific format. Scientific writing is written differently from other types of writing. The results of the exercise or experiment are what are being showcased‚ not the writing. The purpose of scientific writing is not to entertain‚ but to inform. The writing should be simple and easy to understand. There is a specific style that must be followed when writing scientific reports.
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proliferation is limited. Both EMB and SS agars are also differential as they contain lactose‚ which causes indicator dyes in the media to change color in response to increased acidity when it is fermented. On an EMB plate‚ the colony color of a lactose fermenter might range in appearance from intensely pink to purple/black to a metallic green depending on the degree of lactose fermentation. On an SS plate‚ lactose fermenters will appear pink. In addition‚ SS agar contains thiosulfate‚ which if reduced
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of Vibrio cholerae. (TCBS )Thiosulphate Citrate Bile Salts Sucrose is the media used for isolation of Vibrio cholerae 9. Pseudomonas aeruginosa is an oxidase positive‚ non-lactose fermenter. True False 10. For the following Enterobacteriaceae fill in the missing reactions Urea ONPG Lactose Citrate Indole LDC E. coli a. - + b. +
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Next‚ a negative result in the methyl red test indicated that no mixed acid fermentation occurred. The DNase test was performed and yielded a positive result. The SIM test provided two outcomes‚ that the bacterium did not reduce sulfur nor produce indole from tryptophan. Afterwards‚ the bacterium was determined to be positive for lysine decarboxylation and citrate. The purple broth and triple sugar iron tests both indicated gas production. The purple broth test was positive for fermentation‚ and the
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cysteine. This is important in certain environments when cysteine can be used as an energy source for respiration. Any bacteria that could not use cysteine as an energy source were eliminated. The indole production test was used to find out if this species of bacteria could hydrolyze tryptophan into indole‚ pyruvic adic and ammonia. The red layer at the top of the test tube showed a positive test for the unknown species of bacteria indicating that it possess the enzyme tryptophanase. All bacterial
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out until the tip came back out the other end. A notch became visible at this end allowing the tip to be snapped off. The snapped of tip was then used to puncture holes in the plastic film on the tube’s side covering the last eight tests (adonitol‚ lactose‚ arabinose‚ sorbitol‚ Voges-Proskauer‚ dulcitol/PA‚ urea‚ and citrate). Both caps were then replaced‚ and the tube was incubated for 24 hours at 37 °C (Hébert and Leid and Shand
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