Catalase lab report
Arnesh Kaewloyma
9/30/14
Enzyme Catalase under optimum conditions, speeds up the decomposition of hydrogen peroxide The students have studied that Hydrogen peroxide is a jeopardy to metabolism if it’s not obliterated. Enzymes are proteins produced by living cells of that act as catalysts which affects the rate of biochemical reaction by speeds up the breakdown of hydrogen peroxide into water and oxygen gas. The students have formed a hypothesis toward this experiment.
If the speed of decomposition of hydrogen peroxide is related to the temperature then in high temperature it will speeds the decomposition of hydrogen peroxide. Materials ● Catalase
● Hydrogen peroxide 3% and 1%
● Forceps
● Filter paper discs
● Water
● Ice
● Water baths
● Vials
● Marking Pencils
● Stopwatch Procedure
1. Set up an ice bath (0°C), a room temp water bath, a 37°C bath and a boiling water bath
2. Place 5 ml of catalase at 100 units/ml in each 4 test tubes. Place 1 test tube in each of the water baths
3. Place 40 ml 1% H2O2 in each 4 vials. Measure and record the depth of the H2O2.Place 1 vial in the 0°C bath and leave 3 at room temperature. This is necessary because heat will destroy the hydrogen peroxide.
4. Allow the catalase and substrate to incubate at each temperature for about 5 minutes, then test the reaction time at each temperature by dipping a filter into enzyme at the temperature, draining it, and then dropping it into substrate at the same temp. vial of hydrogen peroxide for the boiled catalase. Do not boil hydrogen peroxide. Time how long it takes the filter to rise at each temperature. Record results in the appropriate data chart. Independent Variable : Temperature of enzyme catalase
Dependent Variable : Rate of rising per second
Controlled Variables : H2O2 depth, location, and filter paper
Controlled Group : Water (No H2O2)
How the temperature relates to the rise of filter paper Temperature
H2O2 depth
time
9/30/14
Enzyme Catalase under optimum conditions, speeds up the decomposition of hydrogen peroxide The students have studied that Hydrogen peroxide is a jeopardy to metabolism if it’s not obliterated. Enzymes are proteins produced by living cells of that act as catalysts which affects the rate of biochemical reaction by speeds up the breakdown of hydrogen peroxide into water and oxygen gas. The students have formed a hypothesis toward this experiment.
If the speed of decomposition of hydrogen peroxide is related to the temperature then in high temperature it will speeds the decomposition of hydrogen peroxide. Materials ● Catalase
● Hydrogen peroxide 3% and 1%
● Forceps
● Filter paper discs
● Water
● Ice
● Water baths
● Vials
● Marking Pencils
● Stopwatch Procedure
1. Set up an ice bath (0°C), a room temp water bath, a 37°C bath and a boiling water bath
2. Place 5 ml of catalase at 100 units/ml in each 4 test tubes. Place 1 test tube in each of the water baths
3. Place 40 ml 1% H2O2 in each 4 vials. Measure and record the depth of the H2O2.Place 1 vial in the 0°C bath and leave 3 at room temperature. This is necessary because heat will destroy the hydrogen peroxide.
4. Allow the catalase and substrate to incubate at each temperature for about 5 minutes, then test the reaction time at each temperature by dipping a filter into enzyme at the temperature, draining it, and then dropping it into substrate at the same temp. vial of hydrogen peroxide for the boiled catalase. Do not boil hydrogen peroxide. Time how long it takes the filter to rise at each temperature. Record results in the appropriate data chart. Independent Variable : Temperature of enzyme catalase
Dependent Variable : Rate of rising per second
Controlled Variables : H2O2 depth, location, and filter paper
Controlled Group : Water (No H2O2)
How the temperature relates to the rise of filter paper Temperature
H2O2 depth
time