Chemistry seg. 2
June 15, 2014
05.05 mixtures and solutions: Lab report: outline
Chromatography is the separation of a mixture by passing it in solution or suspension or as a vapor (as in gas chromatography) through a medium in which the components move at different rates. It is used to separate substances. Examples of chromatography are finger-printing, medicines, and separating/ testing histamines. http://www.biotecharticles.com/Agriculture-Article/Applications-of-Chromatography-1299.html
The purpose of this lab is to examine and see the different colors that make up each marker. We will be able to tell by the strips of chromatography paper mixed with salt solution.
Four different markers (including one black permanent marker)
Rubbing alcohol or isopropyl alcohol
Coffee filters (2)
Tall glasses or plastic cups (2)
Clean pitcher or 2-liter bottle
1. Cut the coffee filter into a rectangle measuring three cm by nine cm. Do this twice.
2. Using a pencil (do not use a pen), draw a line one cm (1/2 inch) from the edge of one end of one strip of paper.
3. Make four dots and label which marker is being tested.
4. With the markers, make dots with the different colors and let them dry.
5. Prepare the salt solution by mixing 1/8 teaspoon of salt and three cups of water in a clean pitcher or 2-liter bottle. Stir or shake the solution until it is dissolved.
6. Pour solution into a glass, 1/4th inch high.
7. Tape the strip to a pencil and rest the pencil on top of the jar so that the strip hangs into the jar. The goal is to have the end of the chromatography strip just touching the surface of the solvent solution, with the colored dots above the surface of the liquid. Make sure that the colored spots do not come in direct contact with the liquid in the bottom of the glass.
8. The solution will pass through