Background Information: Paper Chromatography is a separation tool in which pigment is put on a paper made of cellulose and water, and placed in a solvent, in this case isopropyl alcohol. Due to capillary action, the solvent crawls up the paper, separating the pigments. This technique is used to identify components of a mixture, even unknown ones, and can be used to isolate components into pure samples. Real world uses of this technique includes identifying certain biomolecules by pigmenting certain regions of the sample, is used in forensic sciences t analyze DNA and RNA sequencing, and is used in organic chemistry to identify unknown compounds. http://www.tutorvista.com/biology/uses-of-paper-chromatography Purpose: The purpose of this experiment is to compare the separation abilities of salt water and isopropyl alcohol using paper and candy pigments.
Candy with a colored coating, like Skittles® or M&Ms® (4 different colors)
Rubbing alcohol or isopropyl alcohol
Coffee filters (2)
Tall glasses or plastic cups (2)
Foil or paper plate
Toothpicks or cotton swabs (4)
Clean pitcher or 2-liter bottle
1: Cut tin foil is described fashion and gather materials.
2: Cut out the two coffee filters 3cmx9cm.
3: Using a pencil, mark a line that is a half inch from the bottom of both the strips of the filters used.
4: Using a pencil, mark dots a quarter inch apart with the abbreviations of the pigments you will be using.
5: On the tin foil, drop one drop of water at a time and place the skittles or m&ms onto the water to remove the dye.
6: Now put the skittles® on the drops of water to remove the dye.
7: Once the dye is removed put the dye onto your labeled dots via toothpick.
8: While the dye dries, go ahead and mix the water (3 cups) with 1/8 teaspoon of NaCl or table salt.
9: Tape the strips of coffee paper (3cmx9cm) onto the pencils so that they can hang straight